Results 11 to 20 of about 2,501,958 (326)
Development of a 96-Well Plate Dithiothreitol Method for the Colorimetric Determination of Nickel Ions in Water Samples [PDF]
SensorsA new, simple, and accurate assay was developed for the colorimetric determination of nickel ions in 96-well plates. The proposed method utilized the color change immediately visible even to the naked eye during the reaction of Ni(II) ions with ...
George N. Nikas +2 more
doaj +2 more sources
A new cell culture‐based assay quantifies vitamin K 2,3‐epoxide reductase complex subunit 1 function and reveals warfarin resistance phenotypes not shown by the dithiothreitol‐driven VKOR assay [PDF]
Journal of Thrombosis and Haemostasis, 2013 Warfarin directly inhibits the vitamin K 2,3-epoxide reductase complex subunit 1 (VKORC1) enzyme to effect anticoagulation. VKORC1 function has historically been assessed in vitro using a dithiothreitol (DTT)-driven vitamin K 2,3-epoxide reductase (VKOR) assay. Warfarin inhibits wild-type VKORC1 function by the DTT-VKOR assay.
A. Fregin +8 more
semanticscholar +3 more sources
GeoHealth, 2023 Oxidative stress is a mechanism that might raise the toxicity of mineral dust aerosols. We evaluated the oxidative potential (OP) of four reference materials (RMs) of mineral dusts using dithiothreitol assay.
Chiharu Nishita‐Hara +3 more
doaj +2 more sources
Environmental Science & Technology, 2018 We assessed the interactions among the particulate matter (PM) components in generating the reactive oxygen species (ROS) based on a dithiothreitol (DTT) assay. We started with the standard solutions of known redox-active substances, i.e., quinones (9,10-phenanthraquinone, 1,2-naphthoquinone, 1,4-naphthoquinone, and 5-hydroxy-1,4-naphthoquinone) and ...
Haoran Yu +4 more
semanticscholar +3 more sources
Veterinary and Animal Science, 2020 Vitamin K epoxide reductase (VKOR) is a target enzyme for anticoagulants, such as warfarin, that are used as medicines or rodenticides. Assessing VKOR activity is required to ensure the proper usage of these drugs.
Kazuki Takeda +4 more
doaj +4 more sources
Oxidative potential of ambient water-soluble PM 2.5 in the southeastern United States: contrasts in sources and health associations between ascorbic acid (AA) and dithiothreitol (DTT) assays [PDF]
Atmospheric Chemistry and Physics, 2016 . The ability of certain components of particulate matter to induce oxidative stress through the generation of reactive oxygen species (ROS) in vivo may be one mechanism accounting for observed linkages between ambient aerosols and adverse health ...
T. Fang +11 more
semanticscholar +5 more sources
Atmospheric Environment, 2020 Fine particulate matter (PM2.5) air pollution exposure is a leading risk factor for adverse health outcomes, including cardiovascular and respiratory morbidity, and premature mortality. Quantification of PM2.5 oxidative potential (i.e., the ability of PM
K. Berg +5 more
semanticscholar +2 more sources
GeoHealth, 2019 Oxidative potential is an important property of particulate matter (PM) that has been regarded as a more health‐relevant metric than PM mass. We investigated the oxidative potential of size‐segregated PM and effects of Asian dust events in Fukuoka ...
Chiharu Nishita‐Hara +4 more
doaj +2 more sources
Atmosphere, 2022 Atmospheric particulate matter (PM) contains various chemicals, some of which generate in vivo reactive oxygen species (ROS). Owing to their high reactivity and oxidation ability, ROS can cause various diseases.
Yohei Koike, Takayuki Kameda
doaj +1 more source
Sources of PM2.5 Oxidative Potential during Haze and Non-haze Seasons in Chiang Mai, Thailand
Aerosol and Air Quality Research, 2023 Dithiothreitol (DTT) assay is an acellular technique used to investigate the oxidative potential (OP) of chemical substances bound on PM, which may potentially lead to oxidative stress after exposure.
Pitakchon Ponsawansong +9 more
doaj +1 more source