Results 271 to 280 of about 2,501,958 (326)
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Characterization of water-insoluble oxidative potential of PM2.5 using the dithiothreitol assay
Atmospheric Environment, 2020Both water-soluble and insoluble components of ambient particulate matter (PM) have been shown to contribute to the oxidative potential (OP) of PM. In this study, we used the dithiothreitol (DTT) assay to assess the water-soluble (OPWS−DTT) and total OP (
D. Gao +3 more
semanticscholar +2 more sources
Effects of ultrasonic treatment on dithiothreitol (DTT) assay measurements for carbon materials.
Journal of Environmental Sciences, 2019The dithiothreitol (DTT) assay is the most commonly used method to quantify the oxidative potential of fine particles. However, the reported DTT decay rates of carbon black (CB) materials vary greatly among different researchers. This might have resulted
Haotian Jiang +4 more
semanticscholar +3 more sources
Science of The Total Environment, 2019
The exposure to ambient fine particulate matter (PM2.5) can induce oxidative stress, contributing to global burden of diseases. The evaluation of the oxidative potential (OP) of PM2.5 is thus critical for the health risk assessment.
Jingpeng Wang +8 more
semanticscholar +3 more sources
The exposure to ambient fine particulate matter (PM2.5) can induce oxidative stress, contributing to global burden of diseases. The evaluation of the oxidative potential (OP) of PM2.5 is thus critical for the health risk assessment.
Jingpeng Wang +8 more
semanticscholar +3 more sources
A bio-bar-code assay based upon dithiothreitol-induced oligonucleotide release.
Analytical Chemistry, 2005The recently developed bio-bar-code assay for the PCR-less detection of protein and nucleic acid targets has been shown to be extraordinarily sensitive, exhibiting low attomolar sensitivity for protein targets and high zeptomolar sensitivity for nucleic acid targets.
C. Thaxton +4 more
semanticscholar +3 more sources
The use of dithiothreitol in the assay of phenylalanine hydroxylase.
Biochemical Medicine, 1977Abstract An assay for liver phenylalanine hydroxylase based on the nonenzymatic regeneration of 6,7-dimethyltetrahydropterin from 6,7-dimethyldihydropterin (quinonoid form) by dithiothreitol is described. Dithiothreitol not only regenerates the tetrahydropteridine from dyhydropteridine but it also effectively protects the system from peroxide formed ...
C. Bublitz
semanticscholar +3 more sources
Science of The Total Environment, 2019
Excessive generation of reactive oxygen species (ROS) and the corresponding oxidative stress has been recognized as one important cause for the adverse health effects associated with exposure to ambient particulate matter (PM).
Manfei Lin, J. Yu
semanticscholar +4 more sources
Excessive generation of reactive oxygen species (ROS) and the corresponding oxidative stress has been recognized as one important cause for the adverse health effects associated with exposure to ambient particulate matter (PM).
Manfei Lin, J. Yu
semanticscholar +4 more sources
Environmental Pollution, 2019
The cell-free dithiothreitol (DTT) assay is widely used and the DTT consumption rate is interpreted to assess the oxidative potential (OP). Most researchers use an experimental procedure developed by Cho et al.
Manfei Lin, J. Yu
semanticscholar +4 more sources
The cell-free dithiothreitol (DTT) assay is widely used and the DTT consumption rate is interpreted to assess the oxidative potential (OP). Most researchers use an experimental procedure developed by Cho et al.
Manfei Lin, J. Yu
semanticscholar +4 more sources
Endocrinology, 1983
A glucocorticoid receptor exchange assay has been developed for the accurate quantification of both free and steroid-bound receptors in rat liver cytosol. Hepatic cytosol from adrenalectomized rats was saturated in vitro with unlabeled corticosterone. Cytosol was subsequently treated with [3H]dexamethasone (with and without 1000-fold cold dexamethasone)
Kalimi Mohammed, J. Hubbard
semanticscholar +3 more sources
A glucocorticoid receptor exchange assay has been developed for the accurate quantification of both free and steroid-bound receptors in rat liver cytosol. Hepatic cytosol from adrenalectomized rats was saturated in vitro with unlabeled corticosterone. Cytosol was subsequently treated with [3H]dexamethasone (with and without 1000-fold cold dexamethasone)
Kalimi Mohammed, J. Hubbard
semanticscholar +3 more sources
Analytical Biochemistry, 1995
4,4'-Dipyridyl disulfide (4-PDS) and sodium arsenite have been used to develop a rapid and sensitive assay for monothiols in the presence of dithiothreitol (DTT). The procedure is similar to that of Zahler and Cleland (J. Biol. Chem. 243, 716-719, 1968) but involves the use of a lower pH and 4-PDS, which is more effective than 5,5'-dithiobis-(2 ...
M. Le, G. E. Means
semanticscholar +3 more sources
4,4'-Dipyridyl disulfide (4-PDS) and sodium arsenite have been used to develop a rapid and sensitive assay for monothiols in the presence of dithiothreitol (DTT). The procedure is similar to that of Zahler and Cleland (J. Biol. Chem. 243, 716-719, 1968) but involves the use of a lower pH and 4-PDS, which is more effective than 5,5'-dithiobis-(2 ...
M. Le, G. E. Means
semanticscholar +3 more sources
The Analyst, 2011
A simple and efficient electrochemical assay based on self-assembled monolayers (SAMs) was developed for the highly sensitive determination of arsenic(III) in water samples. The synergy of glutathione (GSH), dithiothreitol (DTT) and N-acetyl-L-cysteine (NAC) mixed SAMs enhanced the detection specificity and sensitivity of As(III) in water samples ...
Lingxin Chen +5 more
semanticscholar +3 more sources
A simple and efficient electrochemical assay based on self-assembled monolayers (SAMs) was developed for the highly sensitive determination of arsenic(III) in water samples. The synergy of glutathione (GSH), dithiothreitol (DTT) and N-acetyl-L-cysteine (NAC) mixed SAMs enhanced the detection specificity and sensitivity of As(III) in water samples ...
Lingxin Chen +5 more
semanticscholar +3 more sources