Immobilization of Firefly Bioluminescent System: Development and Application of Reagents
Biosensors, 2022 The present study describes the method of preparing reagents containing firefly luciferase (FLuc) and its substrate, D-luciferin, immobilized into gelatin gel separately or together.
Elena N. Esimbekova +2 more
doaj +1 more source
Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling
Bio-Protocol, 2018 Various environmental stresses or artificial reagents can trigger unfolded protein accumulation in the endoplasmic reticulum (ER) due to the folding capacity of the ER being exceeded.
Yan Bao, Diane Bassham
doaj +1 more source
Characterization of a disease-associated mutation affecting a putative splicing regulatory element in intron 6b of the cystic fibrosis transmembrane conductance regulator (CFTR) gene [PDF]
, 2009 Cystic fibrosis (CF) is a common recessive disorder caused by >1600 mutations in the CF transmembrane conductance regulator (CFTR) gene. About 13% of CFTR mutations are classified as “splicing mutations,” but for almost 40% of these, their role in ...
BAFFICO AM +8 more
core +1 more source
Impact of COVID-19 Lockdown on Oxidative Potential of Particulate Matter: Case of Athens (Greece)
Toxics, 2022 This work evaluates the aerosol oxidative potential (OP) and its changes from modified air pollution emissions during the COVID-19 lockdown period in 2020, with the intent of elucidating the contribution of aerosol sources and related components to ...
Despina Paraskevopoulou +5 more
doaj +1 more source
The Acidic Tail of the Cdc34 Ubiquitin-conjugating Enzyme Functions in Both Binding to and Catalysis with Ubiquitin Ligase SCFC^(dc4*) [PDF]
, 2009 Ubiquitin ligases, together with their cognate ubiquitin-conjugating enzymes, are responsible for the ubiquitylation of proteins, a process that regulates a myriad of eukaryotic cellular functions. The first cullin-RING ligase discovered, yeast SCF^(Cdc4)
Bing Hao +48 more
core +3 more sources
Amino-functionalized poly(l-lactide) lamellar single crystals as a valuable substrate for delivery of HPV16-E7 tumor antigen in vaccine development [PDF]
, 2015 Background: Poly(L-lactide) (PLLA) is a biodegradable polymer currently used in many biomedical applications, including the production of resorbable surgical devices, porous scaffolds for tissue engineering, nanoparticles and microparticles for the ...
Accardi, Luisa +8 more
core +2 more sources
Relationship between redox activity and chemical speciation of size-fractionated particulate matter
Particle and Fibre Toxicology, 2007 Background Although the mechanisms of airborne particulate matter (PM) related health effects remain incompletely understood, one emerging hypothesis is that these adverse effects derive from oxidative stress, initiated by the formation of reactive ...
Cho Arthur K +3 more
doaj +1 more source
Transferrin-polycation conjugates as carriers for DNA uptake into cells. [PDF]
, 1990 We have developed a high-efficiency nucleic acid delivery system that uses receptor-mediated endocytosis to carry DNA macromolecules into cells. We accomplished this by conjugating the iron-transport protein transferrin to polycations that bind nucleic ...
Beug, Hartmut +4 more
core +1 more source
AtmosphereRecent studies indicate that mass concentration alone is not the best parameter to assess the toxicity and the health effects of particulate matter (PM).
Andrea Bergomi +7 more
doaj +1 more source
Airborne and Dermal Collection Methods of Gunshot Residue for Toxicity Studies
Applied Sciences, 2022 Gunshot residue (GSR) has potential negative health effects on humans as a result of inhalation and dermal exposure to the chemical and physical characteristics of GSR such as Pb, Sb, Ba, nitrocellulose, nitroglycerine, and particulate size fraction ...
Samuel Cole Smith +2 more
doaj +1 more source