Results 51 to 60 of about 2,501,958 (326)

cAMP activates adenylate and guanylate cyclase of Dictyostelium discoideum cells by binding to different classes of cell-surface receptors. A study with extracellular Ca2+ [PDF]

, 1985
cAMP induces a transient increase of cAMP and cGMP levels in Dictyostelium discoideum cells. Fast binding experiments reveal three types of cAMP-binding site (S, H and L), which have different off-rates (t0.5, 0.7-15 s) and different affinities (Kd, 15 ...
Brachet, Bumann, De Wit, Devreotes, Dinauer, Gerisch, Gerisch, Janssens, Juliani, Kay, Konijn, Leichtling, Marin, Mato, Pannbacker, Roos, Schaap, Shaffer, Stadel, Toews, Van Haastert, Van Haastert, Van Haastert, Van Haastert, Van Haastert, Van Haastert   +25 more
core   +3 more sources

Protein and cell wall polysaccharide carbonyl determination by a neutral pH 2,4-dinitrophenylhydrazine-based photometric assay

Redox Biology, 2018
A new 2,4-dinitrophenylhydrazine (DNPH)-based photometric assay is developed for the quantification of carbonyls in protein samples from any biological source by protein carbonyl-DNPH hydrazone formation at acidic pH in the presence of denaturing urea ...
Christos D. Georgiou, Dimitrios Zisimopoulos, Vasiliki Argyropoulou, Electra Kalaitzopoulou, George Salachas, Tilman Grune   +5 more
doaj   +1 more source

Technique for Determining the Viability of Acanthamoeba Cysts Treated with a Cysticidal Agent Based on Membrane Integrity

International Journal of Technology, 2021
This study presents a straightforward and reliable method for determining the viability of Acanthamoeba cysts. A standard method for determining Acanthamoeba cyst viability in an in vitro cytotoxicity analysis is required to ensure that the double ...
Nor Farah Azwani Che Mohamad, Nur Syakinah Nafisa Failei, Nurhidayana Mohd Rased, Azila Adnan, Ma Nyuk Ling, Hazlina Ahamad Zakeri, Eny Kusrini, Fatimah Hashim   +7 more
doaj   +1 more source

The IkB kinase inhibitor nuclear factor-kB essential modulator–binding domain peptide for inhibition of balloon injury-induced neointimal formation [PDF]

, 2009
Objective—The activation of nuclear factor-kB (NF-kB) is a crucial step in the arterial wall’s response to injury. The identification and characterization of the NF-kB essential modulator– binding domain (NBD) peptide, which can block the activation of
Carnuccio, R., De Stefano, D., Di Lauro, M.V., Di Meglio, P., Grassia, G., Ialenti, A., Ianaro, A., Kennedy, S., Maddaluno, M., Maffia, P., Musilli, C., Parenti, A., Parratt, C.A.   +12 more
core   +1 more source

Recovery of reduced thiol groups by superoxide-mediated denitrosation of nitrosothiols

Redox Biology, 2022
Nitrosation of critical thiols has been elaborated as reversible posttranslational modification with regulatory function in multiple disorders. Reversibility of S-nitrosation is generally associated with enzyme-mediated one-electron reductions, catalyzed
Stefan Schildknecht, Alex von Kriegsheim, Ksenija Vujacic-Mirski, Fabio Di Lisa, Volker Ullrich, Andreas Daiber   +5 more
doaj   +1 more source

Rapid identification and differentiation of the vaccine strain Rac H from EHV 1 field isolates using a non-radioactive DNA probe [PDF]

, 1992
A method for rapid differentiation between the EHV 1 live vaccine strain Rac H and field isolates is described. Total DNA was isolated from virus-infected small scale cell cultures.
Eichhorn, Werner, Hübert, P. H., Meyer, H., Schwend, C.   +3 more
core   +1 more source

Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [PDF]

, 1983
In the presence of O2 and 5 mM dithiothreitol, penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [P5E·Fe(II)] at 0.5 µ M cleaves pBR322 plasmid DNA (50 µ M in base pairs) on opposite strands to afford discrete DNA fragments as analyzed by agarose gel ...
Dervan, Peter B., Schultz, Peter G.
core  

Development and application of electrochemical dithiothreitol (DTT) assay for analysis of particulate matter

, 2017
Particulate matter (PM) in air pollution, known to have a negative impact on biological systems, is regulated in many countries across the globe. The generation of PM from energy and mining industries is monitored in an effort to minimize its contributions to diminished human health.
Turner, Laurelle Rose, author, Henry, Charles, advisor, Kipper, Matt, committee member, Volckens, John, committee member   +3 more
openaire   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Altered Oxygen Utilisation in Rat Left Ventricle and Soleus after 14 Days, but Not 2 Days, of Environmental Hypoxia. [PDF]

, 2015
The effects of environmental hypoxia on cardiac and skeletal muscle metabolism are dependent on the duration and severity of hypoxic exposure, though factors which dictate the nature of the metabolic response to hypoxia are poorly understood.
Burgess, Sarah L., Horscroft, James A., Hu, Yaqi, Murray, Andrew J.   +3 more
core   +5 more sources