Results 201 to 210 of about 551,983 (304)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Arctic sea-ice ridges are biomass hotspots harboring diverse microbial communities. [PDF]

Commun Earth Environ
Müller O, Gardner J, Olsen LM, Salganik E, Assmy P, Gradinger R, Bratbak G, Hoppe CJM, Lange BA, Muilwijk M, Divine DV, Aberle N, Krause JW, Reigstad M, Leu E, Tsagaraki TM, Larsen A, Høyland KV, Balmonte JP, Boulton W, Dahle H, Eggers L, Fong AA, Guillou G, Lebreton B, Metfies K, Mock T, Petelenz E, Tatarek A, Torres-Valdés S, Torstensson A, Wiktor J, Granskog MA.   +32 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Between gods and green: ritual plant use of traditional festivals in the Chaoshan region, China. [PDF]

J Ethnobiol Ethnomed
Wang J, Zhang S, Deng F, Lin X, Khadka D, Zhong L, Ma B, Ding M, Lee SY, Yu Y, Cui D, Luo B, Shi S.   +12 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

The Analogate of the Divine Physician in the Clinic: Understanding the Christian Health Care Professional as a Symbol of Christ. [PDF]

Linacre Q
Swindeman JP.
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Spiritual wellbeing in advanced cancer patients receiving palliative care in a tertiary cancer centre in northern Kerala, India: a qualitative study. [PDF]

BMJ Open
Biji MS, Kc R, Nair S, Shenoy PK, Spruyt O, Venkateswaran C, B S, Vijayakumar DK.   +7 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

The body before the dance somatic codification, elemental self-regulation, and contemplative recognition in the Naṭarāja tradition. [PDF]

Front Psychol
Samanta S.
europepmc   +1 more source