Results 101 to 110 of about 60,289 (232)

Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing

Small, EarlyView.
The review highlights nature‐inspired nanocarriers for CRISPR delivery, emphasizing viral vectors, extracellular vesicles, liposomes, and lipid nanoparticles. It discusses their roles in improving specificity, minimizing immunogenicity, and overcoming barriers in genome editing. Recent advancements, challenges, and therapeutic applications are explored,
Elizabeth Maria Clarissa, Mamata Karmacharya, Hyunmin Choi, Sumit Kumar, Yoon‐Kyoung Cho   +4 more
wiley   +1 more source

The ecological dichotomy of ammonia-oxidizing archaea and bacteria in the hyper-arid soils of the Antarctic Dry Valleys [PDF]

, 2014
The McMurdo Dry Valleys of Antarctica are considered to be one of the most physically and chemically extreme terrestrial environments on the Earth. However, little is known about the organisms involved in nitrogen transformations in these environments ...
Ana eMachado, Béatrice eFrank-Fahle, Catarina Maria Magalhães, Charles K. Lee, Craig S. Cary, Craig S. Cary   +5 more
core   +3 more sources

TT(N)mGCCTC inhibits archaeal family B DNA polymerases [PDF]

Scientific Reports, 2018
AbstractThe proofreading activity of the archaeal family B DNA polymerases enables PCR with high fidelity. However, thermostable proofreading DNA polymerases occasionally failed to amplify target fragment that could be amplified by Taq DNA polymerase.
Shuhui Sun, Wei Guo, Jin-Shu Yang, Mengsheng Qiu, Xiao-Jing Zhu, Zhong-Min Dai   +5 more
openaire   +2 more sources

The impact of short‐term flooding on soil microbial communities, soil nitrogen and maize productivity in clay loam soils of Ohio, United States

Annals of Applied Biology, EarlyView.
A short‐term flood, implemented in a field experiment with sprinkler irrigation in maize production systems, increased soil moisture levels and reduced autoclaved citrate extractable protein, an indicator of soil biological health that reflects organically bound soil nitrogen, and also corresponded to greater differential abundance of soil bacterial ...
Katherine Naasko, Tvisha Martin, Elena Zakolski, Meredith Mann, Antonino Malacrinò, Wanderson Novais, Alexander Lindsey, Christine Sprunger   +7 more
wiley   +1 more source

Evolution of DNA replication protein complexes in eukaryotes and Archaea. [PDF]

PLoS ONE, 2010
BACKGROUND: The replication of DNA in Archaea and eukaryotes requires several ancillary complexes, including proliferating cell nuclear antigen (PCNA), replication factor C (RFC), and the minichromosome maintenance (MCM) complex.
Nicholas Chia, Isaac Cann, Gary J Olsen
doaj   +1 more source

The return of metabolism: biochemistry and physiology of glycolysis

Biological Reviews, EarlyView.
ABSTRACT Glycolysis is a fundamental metabolic pathway central to the bioenergetics and physiology of virtually all living organisms. In this comprehensive review, we explore the intricate biochemical principles and evolutionary origins of glycolytic pathways, from the classical Embden–Meyerhof–Parnas (EMP) pathway in humans to various prokaryotic and ...
Nana‐Maria Grüning, Federica Agostini, Camila Caldana, Johannes Hartl, Matthias Heinemann, Markus A. Keller, Jan Lukas Krüsemann, Costanza Lamperti, Carole L. Linster, Steffen N. Lindner, Julia Muenzner, Jens Nielsen, Zoran Nikoloski, Bettina Siebers, Jacky L. Snoep, Hezi Tenenboim, Bas Teusink, Spencer J. Williams, Mirjam M. C. Wamelink, Markus Ralser   +19 more
wiley   +1 more source

Overcoming organic and nitrogen overload in thermophilic anaerobic digestion of pig slurry by coupling a microbial electrolysis cell [PDF]

, 2016
The combination of the anaerobic digestion (AD) process with a microbial electrolysis cell (MEC) coupled to an ammonia stripping unit as a post-treatment was assessed both in series operation, to improve the quality of the effluent, and in loop ...
Bonmatí Blasi, August, Cerrillo Moreno, Míriam, Viñas Canals, Marc   +2 more
core   +2 more sources

Adenosine triphosphatases of thermophilic archaeal double-stranded DNA viruses [PDF]

Cell & Bioscience, 2014
Adenosine triphosphatases (ATPases) of double-stranded (ds) DNA archaeal viruses are structurally related to the AAA+ hexameric helicases and translocases. These ATPases have been implicated in viral life cycle functions such as DNA entry into the host, and viral genome packaging into preformed procapsids.
Happonen, Lotta J., Erdmann, Susanne, Garrett, Roger Antony, Butcher, Sarah J   +3 more
openaire   +6 more sources

Evolutionary divergence and functional insights into the heteromeric cis‐prenyltransferase of Paramecium tetraurelia

The FEBS Journal, EarlyView.
Heteromeric cis‐prenyltransferases (CPT) are indispensable for dolichol synthesis and protein N‐glycosylation in most eukaryotes. The catalytic subunits are strongly conserved throughout evolution, in contrast to the evolutionarily variable accessory subunits. The POC1 protein from Paramecium tetraurelia is the smallest identified CPT‐accessory subunit
Agnieszka Onysk, Kamil Steczkiewicz, Mariusz Radkiewicz, Paweł Link‐Lenczowski, Przemysław Surowiecki, Karolina Sztompka, Kariona A. Grabińska, Jacek K. Nowak, Liliana Surmacz   +8 more
wiley   +1 more source

Inteins at Eleven Distinct Insertion Sites in Archaeal Helicase Subunit MCM Exhibit Varied Architectures and Activity Levels Across Archaeal Groups

DNA
Background/Objectives: Inteins are mobile genetic elements invading highly conserved genes across all domains of life and viruses. Five active intein insertion sites (MCM-a through e) had previously been identified and studied in the archaeal replicative
Danielle Arsenault, Gabrielle F. Stack, Johann Peter Gogarten   +2 more
doaj   +1 more source