Differences in sequences between HBV-relaxed circular DNA and covalently closed circular DNA [PDF]
Emerging Microbes and Infections, 2017 The hepatitis B virus (HBV) genome exists in two forms: circular covalently closed DNA (cccDNA) and relaxed circular DNA (RCDNA). Here, we investigated the presence of differences in the sequences of both forms in paired samples of serum and liver tissue.
Magda Rybicka +5 more
doaj +4 more sources
Denaturation of Circular DNA: Supercoil Mechanism
Physical Review E, 2011 The denaturation transition which takes place in circular DNA is analyzed by extending the Poland-Scheraga model to include the winding degrees of freedom.
Alkan Kabakçoğlu +6 more
core +5 more sources
CSA: An efficient algorithm to improve circular DNA multiple alignment [PDF]
BMC Bioinformatics, 2009 Background The comparison of homologous sequences from different species is an essential approach to reconstruct the evolutionary history of species and of the genes they harbour in their genomes.
Pereira Luísa +2 more
doaj +5 more sources
Detection and Validation of Circular DNA Fragments Using Nanopore Sequencing
Frontiers in Genetics, 2022 Occurrence of extra-chromosomal circular DNA is a phenomenon frequently observed in tumor cells, and the presence of such DNA has been recognized as a marker of adverse outcome across cancer types.
Alicia Isabell Tüns +15 more
doaj +1 more source
In vitro amplification of whole large plasmids via transposon-mediated oriC insertion
BioTechniques, 2021 DNA amplification is a fundamental technique in molecular biology. The replication cycle reaction is a new method for amplification of large circular DNA having oriC sequences, which is a replication initiation site of the Escherichia coli chromosome. We
Seia Nara, Masayuki Su'etsugu
doaj +1 more source
Serious overestimation in quantitative PCR by circular (supercoiled) plasmid standard: microalgal pcna as the model gene. [PDF]
PLoS ONE, 2010 Quantitative real-time PCR (qPCR) has become a gold standard for the quantification of nucleic acids and microorganism abundances, in which plasmid DNA carrying the target genes are most commonly used as the standard.
Yubo Hou +3 more
doaj +1 more source
Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus [PDF]
PeerJ, 2022 Background Cyclocytidine hydrochloride (HCl) has been reported to inhibit DNA synthesis by affecting DNA polymerase. Here, we tested the antiviral effect of cyclocytidine on hepatitis B virus (HBV) DNA synthesis, which is reliant on DNA polymerase ...
Xue Wang +7 more
doaj +2 more sources
Extrachromosomal circular DNA: a new potential role in cancer progression
Journal of Translational Medicine, 2021 Extrachromosomal circular DNA (eccDNA) is considered a circular DNA molecule that exists widely in nature and is independent of conventional chromosomes. eccDNA can be divided into small polydispersed circular DNA (spcDNA), telomeric circles (t-circles),
Tianyi Wang +3 more
doaj +1 more source
TeCD: The eccDNA Collection Database for extrachromosomal circular DNA
BMC Genomics, 2023 Background Extrachromosomal circular DNA (eccDNA) is a kind of DNA that widely exists in eukaryotic cells. Studies in recent years have shown that eccDNA is often enriched during tumors and aging, and participates in the development of cell physiological
Jing Guo +4 more
doaj +1 more source
Journal of Hematology & Oncology, 2020 Extrachromosomal circular DNA was recently found to be particularly abundant in multiple human cancer cells, although its frequency varies among different tumor types.
Yuanliang Yan +7 more
doaj +1 more source