Novel Single-Stranded DNA Circular Viruses in Pericardial Fluid of Patient with Recurrent Pericarditis. [PDF]
Emerg Infect Dis, 2016 To the Editor: Circular replication initiation protein (Rep)–encoding single-stranded DNA (ssDNA) (CRESS-DNA) genomes are found in diverse group II virus families, which all possess a conserved Rep-encoding gene and a nonenveloped icosahedral capsid ...
Halary S +8 more
europepmc +2 more sources
Extrachromosomal circular DNA: a new potential role in cancer progression
Journal of Translational Medicine, 2021 Extrachromosomal circular DNA (eccDNA) is considered a circular DNA molecule that exists widely in nature and is independent of conventional chromosomes. eccDNA can be divided into small polydispersed circular DNA (spcDNA), telomeric circles (t-circles),
Tianyi Wang +3 more
doaj +2 more sources
Journal of Hematology & Oncology, 2020 Extrachromosomal circular DNA was recently found to be particularly abundant in multiple human cancer cells, although its frequency varies among different tumor types.
Yuanliang Yan +7 more
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DNA 5-methylcytosine detection and methylation phasing using PacBio circular consensus sequencing
bioRxiv, 2023 Long single-molecular sequencing, such as PacBio circular consensus sequencing (CCS) and nanopore sequencing, is advantageous in detecting DNA 5-methylcytosine (5mC) in CpGs, especially in repetitive genomic regions.
Peng Ni +12 more
semanticscholar +1 more source
Detection and Validation of Circular DNA Fragments Using Nanopore Sequencing
Frontiers in Genetics, 2022 Occurrence of extra-chromosomal circular DNA is a phenomenon frequently observed in tumor cells, and the presence of such DNA has been recognized as a marker of adverse outcome across cancer types.
Alicia Isabell Tüns +15 more
doaj +1 more source
In vitro amplification of whole large plasmids via transposon-mediated oriC insertion
BioTechniques, 2021 DNA amplification is a fundamental technique in molecular biology. The replication cycle reaction is a new method for amplification of large circular DNA having oriC sequences, which is a replication initiation site of the Escherichia coli chromosome. We
Seia Nara, Masayuki Su'etsugu
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Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus [PDF]
PeerJ, 2022 Background Cyclocytidine hydrochloride (HCl) has been reported to inhibit DNA synthesis by affecting DNA polymerase. Here, we tested the antiviral effect of cyclocytidine on hepatitis B virus (HBV) DNA synthesis, which is reliant on DNA polymerase ...
Xue Wang +7 more
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Serious overestimation in quantitative PCR by circular (supercoiled) plasmid standard: microalgal pcna as the model gene. [PDF]
PLoS ONE, 2010 Quantitative real-time PCR (qPCR) has become a gold standard for the quantification of nucleic acids and microorganism abundances, in which plasmid DNA carrying the target genes are most commonly used as the standard.
Yubo Hou +3 more
doaj +1 more source
TeCD: The eccDNA Collection Database for extrachromosomal circular DNA
BMC Genomics, 2023 Background Extrachromosomal circular DNA (eccDNA) is a kind of DNA that widely exists in eukaryotic cells. Studies in recent years have shown that eccDNA is often enriched during tumors and aging, and participates in the development of cell physiological
Jing Guo +4 more
doaj +1 more source
Hepatitis B virus cccDNA is formed through distinct repair processes of each strand
Nature Communications, 2021 HBV covalently closed circular DNA (cccDNA) enables and persists in chronic infection, but the molecular mechanism of its formation is unclear. Here, Wei and Ploss elucidate the detailed kinetics and biochemical steps by which the relaxed circular DNA is
Lei Wei, Alexander Ploss
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