In vitro amplification of whole large plasmids via transposon-mediated oriC insertion
BioTechniques, 2021 DNA amplification is a fundamental technique in molecular biology. The replication cycle reaction is a new method for amplification of large circular DNA having oriC sequences, which is a replication initiation site of the Escherichia coli chromosome. We
Seia Nara, Masayuki Su'etsugu
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DNA-measuring Wadjet SMC ATPases restrict smaller circular plasmids by DNA cleavage
bioRxiv, 2022 Structural maintenance of chromosomes (SMC) complexes fold DNA by loop extrusion to support chromosome segregation, genome maintenance, and gene expression.
Hon Wing Liu +5 more
semanticscholar +1 more source
Serious overestimation in quantitative PCR by circular (supercoiled) plasmid standard: microalgal pcna as the model gene. [PDF]
PLoS ONE, 2010 Quantitative real-time PCR (qPCR) has become a gold standard for the quantification of nucleic acids and microorganism abundances, in which plasmid DNA carrying the target genes are most commonly used as the standard.
Yubo Hou +3 more
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Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus [PDF]
PeerJ, 2022 Background Cyclocytidine hydrochloride (HCl) has been reported to inhibit DNA synthesis by affecting DNA polymerase. Here, we tested the antiviral effect of cyclocytidine on hepatitis B virus (HBV) DNA synthesis, which is reliant on DNA polymerase ...
Xue Wang +7 more
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TeCD: The eccDNA Collection Database for extrachromosomal circular DNA
BMC Genomics, 2023 Background Extrachromosomal circular DNA (eccDNA) is a kind of DNA that widely exists in eukaryotic cells. Studies in recent years have shown that eccDNA is often enriched during tumors and aging, and participates in the development of cell physiological
Jing Guo +4 more
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Hepatitis B virus cccDNA is formed through distinct repair processes of each strand
Nature Communications, 2021 HBV covalently closed circular DNA (cccDNA) enables and persists in chronic infection, but the molecular mechanism of its formation is unclear. Here, Wei and Ploss elucidate the detailed kinetics and biochemical steps by which the relaxed circular DNA is
Lei Wei, Alexander Ploss
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Deciphering extrachromosomal circular DNA in Arabidopsis
Computational and Structural Biotechnology Journal, 2021 Extrachromosomal circular DNA (eccDNA) is independent of the chromosome and exists in many eukaryotes. However, the nature and origin of eccDNA in plants remains unclear.
Keyi Wang +15 more
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Circular Dichroism of DNA [PDF]
European Journal of Biochemistry, 1970 The circular dichroism of sixteen DNA samples from fourteen species has been determined. Plots of molar ellipticity against base composition show considerable scatter, which is much too great to be accounted for in terms of reproducibility of materials and measurements.
W B, Gratzer, L R, Hill, R J, Owen
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DNA-like Helices as Nanosized Polarizers of Electromagnetic Waves
Frontiers in Nanotechnology, 2022 The possibility of using a conducting double DNA-like helix as the basis of an electromagnetic wave polarizer, which converts an incident linearly polarized wave into a reflected wave with circular polarization, has been shown. A high-frequency resonance
Igor V. Semchenko +4 more
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Identification and characterization of extrachromosomal circular DNA in maternal plasma
Proceedings of the National Academy of Sciences of the United States of America, 2020 Significance We observed the presence of extrachromosomal circular DNA (eccDNA) in the plasma of pregnant women. We found that the plasma eccDNA molecules were longer than their linear counterparts. Among such eccDNA molecules, those of fetal origin were
S. Sin +9 more
semanticscholar +1 more source