Results 261 to 270 of about 363,056 (280)

Polyamines, DNA Methylation, and Fungal Differentiation

Critical Reviews in Microbiology, 1994
Mucorales constitute a group of fungi that, because of their growth characteristics, have been used extensively in the study of cell differentiation, cell morphogenesis, and stimuli perception. We have studied the role of polyamine metabolism in the development of different Mucorales, with emphasis on Mucor and Phycomyces species.
openaire   +2 more sources

Fast preparation of fungal DNA for PCR screening

Journal of Microbiological Methods, 2011
Rapid DNA preparation for the quick screening is highly demanded in diverse research fields. Here, we combined an extraction buffer and heat treatment to generate DNA templates from yeast and filamentous fungal materials for PCR. This method may be widely applicable to diverse fungal species in clinical and basic studies.
Kuang-Hung, Liu, Yu-Ling, Yeh, Wei-Chiang, Shen   +2 more
openaire   +2 more sources

A simple protocol for isolation of fungal DNA

Biotechnology Letters, 2003
Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phytopathogen in different regions, and
Sachin R, Tendulkar, Archna, Gupta, Bharat B, Chattoo   +2 more
openaire   +2 more sources

Quantitative Analysis of Fungal DNA in Chronic Rhinosinusitis

The Laryngoscope, 2004
AbstractObjectives/Hypothesis Fungi have been recognized as important pathogens in sinusitis; however, they are equally present in patients with and without sinusitis. The authors postulated that the quantity of fungal DNA in the nose is determinant of disease, is greater in patients with chronic rhinosinusitis, and is directly correlated to their ...
Michael C, Scheuller, Andrew H, Murr, Andrew N, Goldberg, Anand N, Mhatre, Anil K, Lalwani   +4 more
openaire   +2 more sources

Tracking Plant, Fungal, and Bacterial DNA in Honey Specimens*

Journal of Forensic Sciences, 2011
Abstract:  Consuming honey can result in adverse effects owing to poisoning by bacterial (botulism) or plant toxins. We have devised a method to extract polymerase chain reaction (PCR) amplifiable DNA of up to c. 400 bp in length based on dialysis of a 15‐mL honey sample for 18 h against deionized water followed by sequential extraction using phenol ...
OLIVIERI, CRISTINA, MAROTA, Isolina, ROLLO, Franco Ugo, LUCIANI, Stefania   +3 more
openaire   +3 more sources

Allergic fungal rhinosinusitis: detection of fungal DNA in sinus aspirate using polymerase chain reaction

The Journal of Laryngology & Otology, 2009
AbstractObjective:This study investigated allergic fungal rhinosinusitis cases, and aimed to compare the detection of fungi in sinus aspirate by culture and by polymerase chain reaction assay, and to relate the presence of fungi in the nasal sinuses to the type of fungal allergen causing disease.Methods:Sixty-eight cases of allergic fungal ...
S M, El-Morsy, Y W, Khafagy, M M, El-Naggar, A A, Beih   +3 more
openaire   +2 more sources

Preparation of mitochondrial DNA from fungal protoplasts

Journal of Microbiological Methods, 1997
A rapid and simple method based on protoplast formation is presented to prepare mitochondrial DNA (mtDNA) from filamentous fungi. This protocol can be applied to prepare mtDNA from numerous strains in relatively short time.
Zsuzsanna Hamari, Ilona Pfeiffer, Ferenc Kevei, Lajos Ferenczy   +3 more
openaire   +1 more source

Direct extraction of fungal DNA from soil

1996
While diagnostics based on the polymerase chain reaction (PCR) are being applied actively in medical and forensic studies, much less development has occurred with respect to plant pathology and other organisms in the environment (for review see [11]).
Ross N. Nazar, E. Jane Robb, Tatiana Volossiouk   +2 more
openaire   +1 more source

Bioadhesive nanoparticles of fungal chitosan for oral DNA delivery

International Journal of Pharmaceutics, 2010
Chitosan is an ideal candidate for oral DNA delivery due to its mucoadhesive properties. Chitosan (CS) produced under GMP conditions from fungal source was used to encapsulate a plasmid DNA coding for a reporter gene. Nanoparticles made by complex coacervation of CS and DNA had a size around 200 nm, a positive zeta potential, a high association of DNA ...
Laurence, Plapied, Gaëlle, Vandermeulen, Benoît, Vroman, Véronique, Préat, Anne, des Rieux   +4 more
openaire   +2 more sources

DNA isolation from fungal mycelium

2023
Javeed Ahmad Tantray, Sheikh Mansoor, Rasy Fayaz Choh Wani, Nighat Un Nissa   +3 more
openaire   +1 more source