Results 91 to 100 of about 3,265,618 (328)

Cloning, Expression and Toxicity of a Mosquitocidal Toxin Gene of Bacillus thuringiensis subsp. medellin

Memorias do Instituto Oswaldo Cruz, 1997
Bacillus thuringiensis (Bt) subsp. medellin (Btmed) produces parasporal crystalline inclusions which are toxic to mosquito larvae. It has been shown that the inclusions of this bacterium contain mainly proteins of 94, 68 and 28-30 kDa.
Nora Restrepo, Diana Gutierrez, Maria M Patiño, Isabelle Thiéry, Armelle Delécluse, Sergio Orduz   +5 more
doaj   +1 more source

The Mre11-Rad50-Nbs1 complex mediates activation of TopBP1 by ATM [PDF]

, 2009
The activation of ATR-ATRIP in response to double-stranded DNA breaks (DSBs) depends upon ATM in human cells and Xenopus egg extracts. One important aspect of this dependency involves regulation of TopBP1 by ATM.
Abraham R. T., Akiko Kumagai, Andrej Shevchenko, Anna Shevchenko, Bakkenist C. J., Byun T. S., Caldecott K. W., Chapman J. R., Cimprich K. A., Cortez D., Costanzo V., Costanzo V., Cuadrado M., Daniel J. Lew, Delacroix S., Garcia V., Glover J. N., Goldberg M., Guo Z., Hae Yong Yoo, Hashimoto Y., Jazayeri A., Kobayashi T., Kumagai A., Kumagai A., Kumagai A., Kumagai A., Lee J., Lee J. H., Liu Q., Melander F., Mordes D. A., Morishima K., Myers J. S., Nyberg K. A., Parrilla-Castellar E. R., Perry J. A., Qin X. F., Rupnik A., Sancar A., Shevchenko A., Shiloh Y., Spycher C., Stewart G. S., Walter J., Waridel P., William G. Dunphy, Williams R. S., Wu L., Yoo H. Y., Yoo H. Y., Yoo H. Y., Yoo H. Y., You Z., You Z., Zhao H., Zou L.   +56 more
core   +2 more sources

Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.

Proceedings of the National Academy of Sciences of the United States of America, 1986
DNA coding for bacteriophage T7 RNA polymerase was ligated to a vaccinia virus transcriptional promoter and integrated within the vaccinia virus genome.
T. Fuerst, E. G. Niles, F. Studier, B. Moss   +3 more
semanticscholar   +1 more source

Chimeric diphtheria toxin–CCL8 cytotoxic peptide for breast cancer management

Molecular Oncology, EarlyView.
DTCCL8 is a recombinant fusion toxin that targets cancer cells expressing chemokine receptors. By combining diphtheria toxin with CCL8, DTCCL8 binds to multiple receptors on tumor cells and induces selective cytotoxicity. This strategy enables receptor‐mediated targeting of cancer and may support the development of chemokine‐guided therapeutics ...
Bernardo Chavez, Asieh Naderi, Kim‐Tuyen Huynh‐Dam, Vitali Sikirzhytski, Ioulia Chatzistamou, Hippokratis Kiaris   +5 more
wiley   +1 more source

DNA damage, homology-directed repair, and DNA methylation. [PDF]

PLoS Genetics, 2007
To explore the link between DNA damage and gene silencing, we induced a DNA double-strand break in the genome of Hela or mouse embryonic stem (ES) cells using I-SceI restriction endonuclease. The I-SceI site lies within one copy of two inactivated tandem
Concetta Cuozzo, Antonio Porcellini, Tiziana Angrisano, Annalisa Morano, Bongyong Lee, Alba Di Pardo, Samantha Messina, Rodolfo Iuliano, Alfredo Fusco, Maria R Santillo, Mark T Muller, Lorenzo Chiariotti, Max E Gottesman, Enrico V Avvedimento   +13 more
doaj   +1 more source

Olaparib synergy screen reveals Exemestane induces replication stress in triple‐negative breast cancer

Molecular Oncology, EarlyView.
Screening 166 FDA‐approved anticancer drugs identifies the aromatase inhibitor Exemestane as a synergistic partner of PARP inhibitor Olaparib in BRCA‐proficient triple‐negative breast cancer. Exemestane induces ROS‐mediated replication stress, enhancing DNA damage and apoptosis alongside Olaparib.
Nur Aininie Yusoh, Liping Su, Suet Lin Chia, Xiaohe Tian, Haslina Ahmad, Martin R. Gill   +5 more
wiley   +1 more source

CpG DNA is a potent enhancer of specific immunity in mice immunized with recombinant hepatitis B surface antigen.

Journal of Immunology, 1998
Unmethylated CpG dinucleotides in bacterial DNA or synthetic oligodeoxynucleotides (ODN) cause B cell proliferation and Ig secretion, monocyte cytokine secretion, and activation of NK cell lytic activity and IFN-gamma secretion in vivo and in vitro.
H. Davis, Risini Weeranta, T. Waldschmidt, L. Tygrett, J. Schorr, A. Krieg   +5 more
semanticscholar   +1 more source

DNA replication and recombination [PDF]

Nature, 2003
Knowledge of the structure of DNA enabled scientists to undertake the difficult task of deciphering the detailed molecular mechanisms of two dynamic processes that are central to life: the copying of the genetic information by DNA replication, and its reassortment and repair by DNA recombination.
openaire   +3 more sources

Construction of hyperthermostable d‐allulose 3‐epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus

FEBS Open Bio, EarlyView.
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada, Kouhei Ohtani, Pushpa Kiran Gullapalli, Kazuhiko Ishikawa   +3 more
wiley   +1 more source

ArgR is an essential local transcriptional regulator of the arcABC-operon in Streptococcus suis and crucial for biological fitness in acidic environment [PDF]

, 2011
Streptococcus suis is one of the most important pathogens in pigs and can also cause severe infections in humans. Despite its clinical relevance very little is known about the factors contributing to its virulence.
Benga, L., Fulde, M., Goethe, R., Greeff, A., de, Smith, H.E., Valentin-Weigand, P., Willenborg, J.   +6 more
core   +6 more sources