Results 101 to 110 of about 3,265,618 (328)

New Frontiers in the Production of Functional Recombinant Proteins

Bioengineering
Recombinant protein technology, a pivotal subset of biotechnology, facilitates the generation of specific proteins via recombinant DNA techniques, predominantly within heterologous expression systems [...]
Xin Luo, Yi Ma, Qi Xiang
doaj   +1 more source

Mapping the sites of latency and reactivation by bovine herpesvirus 5 (BoHV-5) and a thymidine kinase-deleted BoHV-5 in lambs

Pesquisa Veterinária Brasileira, 2013
A thymidine kinase (tk)-deleted bovine herpesvirus 5 (BoHV-5tkΔ) was previously shown to establish latent infection and reactivate - even poorly - in a sheep model (Cadore et al. 2013). As TK-negative alphaherpesviruses are unlike to reactivate in neural
Gustavo C. Cadore, Gian Marcon, Mario Celso Sperotto Brum, Rudi Weiblen, Eduardo F. Flores   +4 more
doaj   +1 more source

Drf1-dependent Kinase Interacts with Claspin through a Conserved Protein Motif [PDF]

, 2010
The Dbf4/Drf1-dependent kinase (DDK) is required for the initiation of DNA replication in eukaryotes. Another protein, Claspin, mediates the activation of a cellular checkpoint response to stalled replication forks and is also a regulator of replication.
Aparicio, Arias, Chini, Chini, Clarke, Daniel A. Gold, Gambus, Hodgson, Jeong, Jiang, Kastan, Katou, Kim, Kumagai, Kumagai, Kumagai, Kumagai, Kumagai, Kumagai, Lee, Lee, Lin, Lou, Masai, Masai, Melo, Montagnoli, Moyer, Osborn, Pacek, Petermann, Roberts, Sar, Sheu, Silva, Ström, Szyjka, Takahashi, Takahashi, Takeda, Tenca, Tourrière, Tsuji, Tsuji, Walter, William G. Dunphy, Yanow, Yoo, Zou   +48 more
core   +3 more sources

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source

Recombinant vesicular stomatitis viruses from DNA.

Proceedings of the National Academy of Sciences of the United States of America, 1995
We assembled a DNA clone containing the 11,161-nt sequence of the prototype rhabdovirus, vesicular stomatitis virus (VSV), such that it could be transcribed by the bacteriophage T7 RNA polymerase to yield a full-length positive-strand RNA complementary ...
N. Lawson, E. Stillman, M. Whitt, J. Rose   +3 more
semanticscholar   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Plasmid DNA-based Alphavirus Vaccines

Vaccines, 2019
Alphaviruses have been engineered as vectors for high-level transgene expression. Originally, alphavirus-based vectors were applied as recombinant replication-deficient particles, subjected to expression studies in mammalian and non-mammalian cell lines,
Kenneth Lundstrom
doaj   +1 more source

TOPALi: software for automatic identification of recombinant sequences within DNA multiple alignments

Bioinform., 2004
SUMMARY TOPALi is a new Java graphical analysis application that allows the user to identify recombinant sequences within a DNA multiple alignment (either automatically or via manual investigation).
Iain Milne, F. Wright, Glenn Rowe, D. Marshall, D. Husmeier, G. McGuire   +5 more
semanticscholar   +1 more source

Functional parameters of spermatozoa obtained by a new selection device

FEBS Open Bio, EarlyView.
This study compares density gradient centrifugation and the LensHooke CA0™ device for sperm separation. DGC requires multiple steps, specialized equipment, and technical expertise. In contrast, the CA0 device offers key practical and methodological advantages, resulting in a faster, simpler, and more affordable sperm selection method.
Julio C. Chávez, Paulina Torres, Gabriela Carrasquel‐Martínez, María G. Figueroa‐Méndez, Diana Flores, Lina Villar, Israel Maldonado, Marcela B. Trevino, Claudia Treviño   +8 more
wiley   +1 more source

Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate

Indonesian Journal of Biotechnology, 2015
Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the researchwas to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinanttechnology.
Erma Sulistyaningsih, Sukarti Moeljopawiro, Jarot Subandono, Wayan T. Artama   +3 more
doaj