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Role of Recombinant DNA Technology to Improve Life

International Journal of Genomics, 2016
In the past century, the recombinant DNA technology was just an imagination that desirable characteristics can be improved in the living bodies by controlling the expressions of target genes.
Suliman Khan, Muhammad Wajid Ullah, Rabeea Siddique, Ghulam Nabi, Sehrish Manan, Muhammad Yousaf, Hongwei Hou   +6 more
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New Frontiers in the Production of Functional Recombinant Proteins [PDF]

Bioengineering
Recombinant protein technology, a pivotal subset of biotechnology, facilitates the generation of specific proteins via recombinant DNA techniques, predominantly within heterologous expression systems [...]
Xin Luo, Yi Ma, Qi Xiang
doaj   +2 more sources

Newcastle Disease Virus Vectored Chicken Infectious Anaemia Vaccine Induces Robust Immune Response in Chickens

Viruses, 2021
Newcastle disease virus (NDV) strain R2B, with an altered fusion protein cleavage site, was used as a viral vector to deliver the immunogenic genes VP2 and VP1 of chicken infectious anaemia virus (CIAV) to generate a bivalent vaccine candidate against ...
Madhan Mohan Chellappa, Sohini Dey, Dinesh Chandra Pathak, Asmita Singh, Narayan Ramamurthy, Saravanan Ramakrishnan, Asok Kumar Mariappan, Kuldeep Dhama, Vikram N. Vakharia   +8 more
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Serological profiling of rabies antibodies by enzyme-linked immunosorbent assay and its comparative analysis with rapid fluorescent focus inhibition test in mouse model [PDF]

Veterinary World, 2019
Aim: In this study, we have used enzyme-linked immunosorbent assay (ELISA) as an alternative test to replace the cumbersome rapid fluorescent focus inhibition test (RFFIT) to ascertain the immune status of immunized mice against rabies virus ...
Ashis Debnath, Dinesh C. Pathak, Narayan Ramamurthy, Gulam Mohd, A. B. Pandey, Vikramaditya Upmanyu, A. K. Tiwari, R. Saravanan, Madhan Mohan Chellappa, Sohini Dey   +9 more
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Tick-Tattoo: DNA Vaccination Against B. burgdorferi or Ixodes scapularis Tick Proteins

Frontiers in Immunology, 2021
IntroductionBorrelia burgdorferi sensu lato (sl) is the causative agent of Lyme borreliosis. Currently there is no human vaccine against Lyme borreliosis, and most research focuses on recombinant protein vaccines. DNA tattoo vaccination with B.
Michelle J. Klouwens, Michelle J. Klouwens, Michelle J. Klouwens, Jos J. A. Trentelman, Alex Wagemakers, Jasmin I. Ersoz, Adriaan D. Bins, Adriaan D. Bins, Joppe W. Hovius, Joppe W. Hovius, Joppe W. Hovius   +10 more
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An easy method to generate recombinant pseudorabies virus expressing the capsid protein of Porcine circovirus type 2d

Frontiers in Microbiology, 2023
IntroductionHomologous recombination is an effective way to generate recombinant viruses for vaccine research such as pseudorabies virus (PRV) and adenovirus.
Jingqiang Ren, Jingqiang Ren, Jingqiang Ren, Rachel Madera, Chase Cunningham, Jishu Shi, Lihua Wang   +6 more
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Recombinant Newcastle Disease Virus (NDV) Expressing Sigma C Protein of Avian Reovirus (ARV) Protects against Both ARV and NDV in Chickens

Pathogens, 2019
Newcastle disease (ND) and avian reovirus (ARV) infections are a serious threat to the poultry industry, which causes heavy economic losses. The mesogenic NDV strain R2B is commonly used as a booster vaccine in many Asian countries to control the disease.
Deep Prakash Saikia, Kalpana Yadav, Dinesh C. Pathak, Narayan Ramamurthy, Ajai Lawrence D’Silva, Asok Kumar Marriappan, Saravanan Ramakrishnan, Vikram N. Vakharia, Madhan Mohan Chellappa, Sohini Dey   +9 more
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A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Nucleic Acids Research, 1979
A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction ...
H. Birnboim, J. Doly
semanticscholar   +1 more source

Newcastle Disease Virus Vectored Bivalent Vaccine against Virulent Infectious Bursal Disease and Newcastle Disease of Chickens

Vaccines, 2017
Newcastle disease virus (NDV) strain F is a lentogenic vaccine strain used for primary vaccination in day-old chickens against Newcastle disease (ND) in India and Southeast Asian countries.
Sohini Dey, Madhan Mohan Chellappa, Dinesh C. Pathak, Satish Gaikwad, Kalpana Yadav, Saravanan Ramakrishnan, Vikram N. Vakharia   +6 more
doaj   +1 more source

Staphylococcus aureus Protection-Related Type 3 Cell-Mediated Immune Response Elicited by Recombinant Proteins and GM-CSF DNA Vaccine

Vaccines, 2021
Staphylococcus aureus mastitis remains a major challenge for dairy farming. Here, 24 mice were immunized and divided into four groups: G1: control; G2: Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) DNA vaccine; G3: F0F1 ATP synthase subunit α
Kamila R. Santos, Fernando N. Souza, Eduardo M. Ramos-Sanchez, Camila F. Batista, Luiza C. Reis, Wesley F. Fotoran, Marcos B. Heinemann, Hiro Goto, Magnus Gidlund, Adriano F. Cunha, Angélica Rosa Faria, Hélida M. Andrade, Andrey P. Lage, Mônica M. O. P. Cerqueira, Alice M. M. P. Della Libera   +14 more
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