Results 241 to 250 of about 888,381 (281)
Oral delivery of GLP-1 analogues by recombinant Lactococcus lactis restores pancreatic islet structure through intestinal mucosal absorption in diabetic mice. [PDF]
EBioMedicineHuang Y +13 more
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Rye sprout as a host for the rapid transient production of a recombinant protein using a geminivirus DNA-containing expression vector. [PDF]
Plant Biotechnol (Tokyo)Kitajima S, Morita S, Natsuhara K.
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Synaptic potentiation requires PARP1 activation: prevailing concepts are revisited. [PDF]
Mol PsychiatryCohen-Armon M, Segal M.
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Cold Spring Harbor Protocols, 2023
The method described here allows editing of the bacterial genome without leaving any secondary changes (scars) behind. This method uses a tripartite selectable and counterselectable cassette comprising an antibiotic-resistance gene (catorkan) and thetetRrepressor gene linked to a Ptetpromoter-ccdBtoxin gene fusion.
Nara Figueroa-Bossi +2 more
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The method described here allows editing of the bacterial genome without leaving any secondary changes (scars) behind. This method uses a tripartite selectable and counterselectable cassette comprising an antibiotic-resistance gene (catorkan) and thetetRrepressor gene linked to a Ptetpromoter-ccdBtoxin gene fusion.
Nara Figueroa-Bossi +2 more
openaire +2 more sources
DNA Recombineering Applications
Cold Spring Harbor Protocols, 2023The ability to manipulate the bacterial genome is an obligatory premise for the study of gene function and regulation in bacterial cells. The λ red recombineering technique allows modification of chromosomal sequences with base-pair precision without the need of intermediate molecular cloning steps.
Nara Figueroa-Bossi +2 more
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The Lancet, 1981
Publisher Summary Plasmids are widely used as vectors and methods of their isolation can vary depending upon the host organism. On the basis of phenotypic effect and organization in the chromosomes, genes can be subdivided into various groups, such as simple genes, complex genes, operons, regulons, and multiple regulons.
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Publisher Summary Plasmids are widely used as vectors and methods of their isolation can vary depending upon the host organism. On the basis of phenotypic effect and organization in the chromosomes, genes can be subdivided into various groups, such as simple genes, complex genes, operons, regulons, and multiple regulons.
openaire +4 more sources
Introduction to recombinant DNA
Journal of Inherited Metabolic Disease, 1986AbstractThis paper describes the current state of knowledge of methods for analysing gene structure and localization. Illustrations are given of the preparation of complementary DNA libraries and their screening by positive‐negative selection, the use of synthetic oligodeoxynucleotides and the use of antibodies. Analysis of the EGF precursor is used as
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