Results 281 to 290 of about 3,265,618 (328)
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Introduction to recombinant DNA
Journal of Inherited Metabolic Disease, 1986AbstractThis paper describes the current state of knowledge of methods for analysing gene structure and localization. Illustrations are given of the preparation of complementary DNA libraries and their screening by positive‐negative selection, the use of synthetic oligodeoxynucleotides and the use of antibodies. Analysis of the EGF precursor is used as
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Cold Spring Harbor Protocols, 2023
The method described here allows editing of the bacterial genome without leaving any secondary changes (scars) behind. This method uses a tripartite selectable and counterselectable cassette comprising an antibiotic-resistance gene (catorkan) and thetetRrepressor gene linked to a Ptetpromoter-ccdBtoxin gene fusion.
Nara Figueroa-Bossi +2 more
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The method described here allows editing of the bacterial genome without leaving any secondary changes (scars) behind. This method uses a tripartite selectable and counterselectable cassette comprising an antibiotic-resistance gene (catorkan) and thetetRrepressor gene linked to a Ptetpromoter-ccdBtoxin gene fusion.
Nara Figueroa-Bossi +2 more
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2003
Publisher Summary Recombinant DNA is the method of joining two or more DNA molecules to create a hybrid. The technology is made possible by two types of enzymes, restriction endonucleases and ligase. A restriction endonuclease recognizes a specific sequence of DNA and cuts within, or close to, that sequence.
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Publisher Summary Recombinant DNA is the method of joining two or more DNA molecules to create a hybrid. The technology is made possible by two types of enzymes, restriction endonucleases and ligase. A restriction endonuclease recognizes a specific sequence of DNA and cuts within, or close to, that sequence.
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1978
The debate over recombinant DNA research is a unique event, perhaps a turning point, in the history of science. For the first time in modern history there has been widespread public discussion about whether and how a promising though potentially dangerous line of research shall be pursued.
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The debate over recombinant DNA research is a unique event, perhaps a turning point, in the history of science. For the first time in modern history there has been widespread public discussion about whether and how a promising though potentially dangerous line of research shall be pursued.
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Recombination in yeast and the recombinant DNA technology
Genome, 1989The development of methods to isolate eukaryotic genes, alter these genes in vitro and reintroduce them into the cell has had a major impact on the study of recombination in the yeast Saccharomyces cerevisiae. In this paper we discuss how recombinant DNA techniques have been employed in the study of recombination in yeast and the results that have ...
Martin Kupiec +9 more
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The recombinant DNA technology
Journal of Veterinary Pharmacology and Therapeutics, 1983The recombinant DNA technology or DNA cloning permits the isolation, amplification, and precise manipulation of specific DNA fragments. This is generally accomplished by linking or recombining the desired DNA fragment with a DNA molecule, termed the vector, which is capable of directing the replication of itself in a suitable host cell and any DNA ...
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DNA Recombination by XPCR [PDF]
, 2006 The first step of the Adleman-Lipton extract model in DNA computing is the combinatorial generation of libraries. In this paper a new method is proposed for generating a initial pool, it is a quaternary recombination of strings via application of null context splicing rules.
FRANCO, Giuditta +3 more
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Recombinant DNA and the pediatrician
The Journal of Pediatrics, 1981It is our opinion that the basic science clearly explained and illustrated in this article is surely worth understanding, in view of the several advances already established, the prospects which are imminent, and probably many other evolutions not yet anticipated.
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Recombination of DNA Molecules
1966Publisher Summary The chapter discusses the recombination of deoxyribonucleic acid (DNA) molecules. The chapter also discusses the major features of genetic recombination and also summarizes some current speculations on the recombination of DNA molecules.
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