Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells [PDF]
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells.
Apostolaki+47 more
core +1 more source
Isolation and characterization of Brochothrix phage ADU4
B. thermosphacta is a psychrotrophic bacterium that often forms the predominant part of the spoilage microflora of aerobically and anaerobically stored meats.
Abdulkerim Karaynir+4 more
doaj
Sustained thrombolysis with DNA-recombinant tissue type plasminogen activator in rabbits.
Tissue type plasminogen activator (t-PA) is an effective thrombolytic agent in experimental animals. The duration of the thrombolytic effect of infused t-PA is unknown.
G. Agnelli+4 more
semanticscholar +1 more source
Basidiomycetous fungi, Ganoderma lucidum MDU-7 and Ganoderma sp. kk-02 secreted multiple laccase isozymes under diverse growth condition. Aromatic compounds and metal salts were also found to regulate the differential expression of laccase isozymes from ...
Krishna K. Sharma+7 more
doaj +1 more source
Ciz1 cooperates with cyclin-A-CDK2 to activate mammalian DNA replication in vitro [PDF]
Initiation of mammalian DNA replication can be reconstituted from isolated G1-phase nuclei and cell extracts, supplemented with cyclin-dependent protein kinases (CDKs).
Ainscough+30 more
core +3 more sources
A Very Strong Enhancer Is Located Upstream of an Immediate Early Gene of Human Cytomegalovirus [PDF]
A strong transcription enhancer was identified in the genomic DNA (235 kb) of human cytomegalovirus (HCMV), a ubiquitous and severe pathogen of the herpesvirus group.
Boshart, Michael+5 more
core +1 more source
Investigating BB0405 as a novel Borrelia afzelii vaccination candidate in Lyme borreliosis
BB0405 is a surface exposed Borrelia burgdorferi protein and its vaccination protected mice against B. burgdorferi infection. As BB0405 is highly conserved across different B.
M. J. Klouwens+8 more
doaj +1 more source
MRE11 facilitates the removal of human topoisomerase II complexes from genomic DNA [PDF]
Topoisomerase II creates a double-strand break intermediate with topoisomerase covalently coupled to the DNA via a 5'-phosphotyrosyl bond. These intermediate complexes can become cytotoxic protein-DNA adducts and DSB repair at these lesions requires ...
Austin, Caroline+10 more
core +3 more sources
A DNA recombinant database management system
A set of computer programs is described which constitutes a clone database management system. Maintenance of the database and the stocks of material is designed to be under the control of one person or group of people, who may insert, delete or modify ...
C. Tolstoshev+3 more
semanticscholar +1 more source
Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate
Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the research was to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinant technology.
Erma Sulistyaningsih+3 more
doaj +1 more source