Results 51 to 60 of about 2,933,423 (207)
A multi-landing pad DNA integration platform for mammalian cell engineering
Nucleic Acids Research, 2018 Engineering mammalian cell lines that stably express many transgenes requires the precise insertion of large amounts of heterologous DNA into well-characterized genomic loci, but current methods are limited. To facilitate reliable large-scale engineering
L. Gaidukov +16 more
semanticscholar +1 more source
Pesquisa Veterinária Brasileira, 2013 The ability of thymidine kinase (tk)-deleted recombinant bovine herpesvirus 5 (BoHV-5tkΔ) to establish and reactivate latent infection was investigated in lambs. During acute infection, the recombinant virus replicated moderately in the nasal mucosa, yet
Gustavo C. Cadore +5 more
doaj +1 more source
Kinetics of protein-DNA interaction: facilitated target location in sequence-dependent potential [PDF]
Biophys. J. 87:4021-4035 (2004), 2004 Recognition and binding of specific sites on DNA by proteins is central for many cellular functions such as transcription, replication, and recombination. In the process of recognition, a protein rapidly searches for its specific site on a long DNA molecule and then strongly binds this site.
arxiv +1 more source
PLoS ONE, 2010 Background Induction of HIV-1-specific T-cell responses relevant to diverse subtypes is a major goal of HIV vaccine development. Prime-boost regimens using heterologous gene-based vaccine vectors have induced potent, polyfunctional T cell responses in ...
R. Koup +12 more
semanticscholar +1 more source
VacciMonitor, 2021 The aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors.
Sobirdjan A. Sasmakov +7 more
doaj
Communications Biology, 2022 DNA methyltransferase 3A (DNMT3A) is shown to efficiently methylate recombinant mononucleosome linker DNA and a role for H3K36me3 in the stimulation of DNA methylation is revealed that is independent of its interaction with the PWWP domain.
Alexander Bröhm +8 more
doaj +1 more source
Analytical study of the effect of recombination on evolution via DNA shuffling [PDF]
, 2003 We investigate a multi-locus evolutionary model which is based on the DNA shuffling protocol widely applied in \textit{in vitro} directed evolution. This model incorporates selection, recombination and point mutations. The simplicity of the model allows us to obtain a full analytical treatment of both its dynamical and equilibrium properties, for the ...
arxiv +1 more source
Strategies of Loop Recombination in Ciliates [PDF]
Discrete Applied Mathematics, v. 156, 1736-1753, 2008, 2006 Gene assembly in ciliates is an extremely involved DNA transformation process, which transforms a nucleus, the micronucleus, to another functionally different nucleus, the macronucleus. In this paper we characterize which loop recombination operations (one of the three types of molecular operations that accomplish gene assembly) can possibly be applied
arxiv +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 1996 An efficient method of constructing recombinant adenoviruses (Ads) has been established. The expression unit to be introduced into recombinant Ad was first inserted into the unique Swa I site of the full-length Ad genome cloned in a cassette cosmid.
S. Miyake +7 more
semanticscholar +1 more source
Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate
Indonesian Journal of Biotechnology, 2005 Rhoptry protein belongs to an excretory and secretory antigens (ESAs) that play an important role during active penetration of parasite into the cell target.
Wayan T. Artama +4 more
doaj +1 more source