Results 81 to 90 of about 2,933,423 (207)

Predicting Knot and Catenane Type of Products of Site-specific Recombination on Twist Knot Substrates [PDF]

open access: yesarXiv, 2010
Site-specific recombination on supercoiled circular DNA molecules can yield a variety of knots and catenanes. Twist knots are some of the most common conformations of these products and they can act as substrates for further rounds of site-specific recombination. They are also one of the simplest families of knots and catenanes.
arxiv  

Enhanced specific immune responses by CpG DNA in mice immunized with recombinant hepatitis B surface antigen and HB vaccine

open access: yesVirology Journal, 2011
Background Hepatitis B vaccine adjuvant, alum, is generally used for vaccination although it does not stimulate Th1 immunity and 10% of the population has low or no antibody response.
Wang Xingtai   +4 more
doaj   +1 more source

Polynomial identities for ternary intermolecular recombination [PDF]

open access: yesarXiv, 2010
The operation of binary intermolecular recombination, originating in the theory of DNA computing, permits a natural generalization to n-ary operations which perform simultaneous recombination of n molecules. In the case n = 3, we use computer algebra to determine the polynomial identities of degree <= 9 satisfied by this trilinear nonassociative ...
arxiv  

Boosting with recombinant vaccinia increases immunogenicity and protective efficacy of malaria DNA vaccine.

open access: yesProceedings of the National Academy of Sciences of the United States of America, 1998
To enhance the efficacy of DNA malaria vaccines, we evaluated the effect on protection of immunizing with various combinations of DNA, recombinant vaccinia virus, and a synthetic peptide.
M. Sedegah   +6 more
semanticscholar   +1 more source

Hypothesis: Homologous recombination depends on parallel search [PDF]

open access: yesarXiv, 2016
It is not known how a cell manages to find a specific DNA sequence sufficiently fast to repair a broken chromosome through homologous recombination. I propose that the solution is based on a parallelized search implemented by freely diffusing molecules programmed with sequences corresponding to those flanking the break site.
arxiv  

Recombinant Human DNA (Cytosine-5) Methyltransferase

open access: yesJournal of Biological Chemistry, 1999
A method is described to express and purify human DNA (cytosine-5) methyltransferase (human DNMT1) using a protein splicing (intein) fusion partner in a baculovirus expression vector. The system produces ∼1 mg of intact recombinant enzyme >95% pure per 1.
S. Pradhan   +3 more
semanticscholar   +1 more source

Cloning and Sequencing cDNA Encoding for Rhoptry-2 Toxoplasma Gondii Tachyzoite Local Isolate

open access: yesIndonesian Journal of Biotechnology, 2015
Rhoptry protein belongs to an excretory and secretory antigens (ESAs) that play an important role during activepenetration of parasite into the cell target. This protein an able Toxoplasma gondii to actively penetrate targetedcell, meanwhile ESAs protein
Wayan T. Artama   +4 more
doaj   +2 more sources

Characterization and genomic analysis of PA-56 Pseudomonas phage from Istanbul, Turkey: Antibacterial and antibiofilm efficacy alone and with antibiotics

open access: yesHeliyon
Phages are ubiquitous in freshwater, seawater, soil, the human body, and sewage water. They are potent biopharmaceuticals against antimicrobial-resistant bacteria and offer a promising alternative for treating infectious diseases.
Damla Damar Celik   +4 more
doaj  

Detection of Small Sequence Differences Using Competitive PCR: Molecular Monitoring of Genetically Improved, Mercury-Reducing Bacteria

open access: yesBioTechniques, 2001
A quantitative PCR approach is presented to detect small genomic sequence differences for molecular quantification of recombinant DNA. The only unique genetic feature of the mercury-reducing, genetically improved Pseudomonas putida KT2442::mer73 ...
A. Felske   +6 more
doaj   +1 more source

Nonlocal Structural Effects of Water on DNA Homology Recognition [PDF]

open access: yes
The mechanism behind mutual recognition of homologous DNA sequences prior to genetic recombination is one of the remaining puzzles in molecular biology. Leading models of homology recognition, based on classical electrostatics, neglect the short-range nonlocal screening effects arising from structured water around DNA, and hence may only provide ...
arxiv   +1 more source

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