Results 191 to 200 of about 410,825 (308)

Engineering a Thermostable Reverse Transcriptase for RT-PCR Through Rational Design of <i>Pyrococcus furiosus</i> DNA Polymerase. [PDF]

Biomolecules
Kuznetsova AA, Grishina IA, Mikushina ES, Kuznetsov NA.   +3 more
europepmc   +1 more source

Embedded CRISPRi Enhances Gene‐Silencing Efficiency in Drosophila

Advanced Science, EarlyView.
Current CRISPR interference (CRISPRi) technology in Drosophila has limited efficiency. This study introduces the emCRISPRi platform, which significantly enhances transcriptional silencing efficacy by embedding inhibitory domains within the dCas9 architecture.
Pengchong Fu, Xuedi Zhang, Ying Zhou, Jie Zheng, Angyang Sun, Kailong Zhuang, Weiwei Bao, Guanjun Gao   +7 more
wiley   +1 more source

Cloning-Free Genome Editing by CRISPR/T7RNAP/Cas9 in Trypanosoma cruzi. [PDF]

Methods Mol Biol
Chiurillo MA, Ahmed M, González C, Rosón JN, Das A, Lander N.   +5 more
europepmc   +1 more source

A Tac1‐Expressing Brainstem Pathway Underlies the Pathogenesis of Trigeminal Neuralgia

Advanced Science, EarlyView.
A critical TG‐Sp5CTac1‐PBNTac1 pathway drives trigeminal neuropathic pain (TNP). Tac1‐expressing parabrachial nucleus (PBNTac1) neurons exhibit heightened responses to innocuous stimuli in TNP, and chemogenetic inhibition of these neurons effectively prevents TNP development.
Liting Sun, Jia‐Jia Wang, Xiang‐Yu Li, Xin‐Yi Lin, Juan Li, Qiu‐Tong Yu, Zi‐Han Wang, Xue‐Ping Gao, Lei Jin, Wei‐Ke Li, Tian‐Lin Cheng, Juan Deng   +11 more
wiley   +1 more source

Epigenetic odyssey to decrypt the hidden code for sustainable brassica production: enhancing yield, stress resilience and nutritional quality. [PDF]

Front Plant Sci
Maithreyi RS, Preet S, Tanwar K, Chakraborty P, Gupta I, Verma R, Bharti J, Thangaraj A, Kaul R, Kaul T.   +9 more
europepmc   +1 more source

Causal Prediction of TP53 Variant Pathogenicity Using a Perturbation‐Informed Protein Language Model

Advanced Science, EarlyView.
A TP53‐specific predictor, CaVepP53, is developed by fine‐tuning ESMC on experimentally validated variants, quantifying pathogenicity via Euclidean distances. It outperforms general‐purpose models and extends to five cancer genes, enabling interpretable variant classification for precision medicine.
Huiying Chen, Yang Zhao, Boqiang Hu, Wuke Wang, Minfang Song, Annabeth Xinyu Zhao, Xiangyang Li, Gefei Wang, Yanfen Wang, Weiyan Zheng, Xinpeng Zhang, Xia Lin, Yanbin Yin, Xingxu Huang, Jinfang Zheng, Tingbo Liang   +15 more
wiley   +1 more source

Machine learning-optimized long single-stranded DNA synthesis technology empowers high-precision diagnostic-therapeutic integration in living cells. [PDF]

Nucleic Acids Res
Zhang Y, Xu Y, Ding Z, Cheng Y, Gao Y, Ye J, Zhang H, Li P, Wu H.   +8 more
europepmc   +1 more source

Single‐Cell Transcriptomics Reveals FLS2‐Dependent Hypoxia Signaling and ERF13‐Mediated Transcription During flg22‐Triggered Immunity

Advanced Science, EarlyView.
This study employs sc‐RNA sequencing, genetics, and phenotyping to systematically map the cell‐type‐specific immune responses triggered by flg22. It reveals FLS2‐dependent transcriptional reprogramming in epidermal and mesophyll cells, and uncovers crosstalk between immune and hypoxia signaling pathways.
Yaping Zhou, Aizhi Qin, Mengfan Li, Qianli Zhao, Luyao Kong, Lulu Yan, Chunyang Li, Hao Liu, Yinpeng Zhang, Jiani Long, Mengyu Liao, Mengmeng Zhou, Xiaoli Fan, Baozhen Wang, Wenxuan Kang, Shui Wang, Zhixin Liu, Xuwu Sun   +17 more
wiley   +1 more source

Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach. [PDF]

STAR Protoc
Clairoux A, Rivera M, McKeague M, Mittermaier A.   +3 more
europepmc   +1 more source

Review 1 - Nature (Busby) [PDF]

, 2009
Scarlato, Vincenzo
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