Results 261 to 270 of about 48,433 (280)

The DNA-directed RNA polymerases of soybean

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
Abstract Four cellular RNA polymerases have been partially purified from hypocotyls of soybean ( Glycine max ). Enzyme isolation from nuclear preparations indicate the DEAE polymerases I and II are localized within the nucleus. Inhibitor studies showed that DEAE polymerase II was preferentially inhibited by α-amanitin, DEAE peaks III and IV were ...
Joe L. Key, Paul A. Horgen
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On the Binding of Rifampicin to the DNA‐Directed RNA Polymerase from Escherichia coli

European Journal of Biochemistry, 1973
The capacity of RNA polymerase to bind the antibiotic rifampicin is rather sensitive to conditions which lead to conformational changes in the enzyme. Repeated freezing and thawing in 1 M LiCl as well as treatment with guanidinium chloride destroys the binding affinity of the enzyme for the inhibitor. Similarly rifampicin does not associate with any of
Guido R. Hartmann, Ute Lill
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Effect of granaticin on the activity of DNA-directed RNA polymerase fromStreptomyces granaticolor

Folia Microbiologica, 1989
DNA-directed RNA polymerase was isolated from wild-type and mutant (asporogenic and granaticin overproducing) Streptomyces granaticolor. The effect of granaticin on the enzyme activity was compared with that of standard transcription inhibitors, rifampicin and rifamycin SV and shown to be zero.
Spízek J, M. Klégr, J. Šmardová, J Stastná   +3 more
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Mode of inhibition of HIV-1 reverse transcriptase by polyacetylenetriol, a novel inhibitor of RNA- and DNA-directed DNA polymerases [PDF]

Biochemical Journal, 2002
Polyacetylenetriol (PAT), a natural marine product from the Mediterranean sea sponge Petrosia sp., was found to be a novel general potent inhibitor of DNA polymerases. It inhibits equally well the RNA- and DNA-dependent DNA polymerase activities of retroviral reverse transcriptases (RTs) (i.e.
Amnon Hizi, Amira Rudi, Shoshana Loya, Yoel Kashman   +3 more
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DNA-directed RNA polymerase from HeLa cells

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
Abstract DNA-directed RNA polymerase was solubilized from total HeLa cells. Three distinct classes of the enzyme could be clearly differentiated by their sensitivity toward α-amanitin. While form A is completely resistant to high concentrations (133 μg/ml) of this toxin, enzyme B is highly sensitive and is completely inhibited by concentrations of 0 ...
Bernd J. Benecke, Klaus H. Seifart
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The isolation and properties of a DNA-directed RNA polymerase from yeast mitochondria

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
A method is described for the rapid isolation of yeast mitochondrial DNA-directed RNA polymerase. The enzyme obtained had a specific activity of 1.56 nmol UMP incorporated per mg protein in 20 min at 37 degrees C, and is some 95% pure. This purified enzyme upon polyacrylamide gel elctrophoresis consists of a single polypeptide of 68 000 mol.
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Improved prediction of prognosis and therapy response for lung adenocarcinoma after identification of DNA-directed RNA polymerase-associated lncRNAs

Journal of Cancer Research and Clinical Oncology, 2023
DNA-directed RNA polymerase (DDRP) related genes and long non-coding RNAs (lncRNAs) play an important role in the development of lung adenocarcinoma (LUAD), the leading cause of cancer-related death worldwide. Therefore, we aimed to construct a DDRP-associated lncRNA model to predict the prognosis of LUAD and to evaluate its sensitivity to ...
Jiaao, Yu, Liqiang, Lan, Caixin, Liu, Xiao, Zhu   +3 more
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Mitochondrial DNA-directed RNA polymerase from Saccharomyces cerevisae mitochondria

Biochemical and Biophysical Research Communications, 1971
Abstract A DNA-directed RNA polymerase activity has been detected in yeast mitochondrial extracts which is sensitive to rifampicin. This activity is distinct from that found in the nucleus and cytoplasm, and is possibly coded for, or under the control of the mitochondrial genome.
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The effect of aryl hydrocarbon receptor ligands on the expression of polymerase (DNA directed) kappa (Polκ), polymerase RNA II (DNA directed) polypeptide A (PolR2a), CYP1B1 and CYP1A1 genes in rat liver

Environmental Toxicology and Pharmacology, 2012
The aryl hydrocarbon receptor (AhR) mediates a variety of biological responses to ubiquitous environmental pollutants. AhR is ligand activated transcription factor with high affinities for aromatic planar compounds such as β-naphthoflavone (BNF), 3-methylcholanthrene (3-MC), benzo[a]pyrene (BaP) or dioxin (TCDD).
Agnieszka Anna Rawłuszko, Damian Brauze
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Lysine Methylation Mapping of Crenarchaeal DNA-Directed RNA Polymerases by Collision-Induced and Electron-Transfer Dissociation Mass Spectrometry

Journal of Proteome Research, 2014
Enzymatic machineries fundamental for information processing (e.g., transcription, replication, translation) in Archaea are simplified versions of their eukaryotic counterparts. This is clearly noticeable in the conservation of sequence and structure of corresponding enzymes (see for example the archaeal DNA-directed RNA polymerase (RNAP)). In Eukarya,
Mikel Azkargorta, Magdalena N. Wojtas, Nicola G. A. Abrescia, Felix Elortza   +3 more
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