Results 101 to 110 of about 35,302 (251)

A comparative assessment of 16S ribosomal RNA and Cytochrome C Oxidase Subunit I (COI) Primers for Amphibian DNA Barcoding [PDF]

open access: yesBIO Web of Conferences
Amphibians, a diverse and ecologically important group, are facing global declines due to various factors, including habitat loss and climate change. Accurate species identification is crucial for effective conservation efforts, and DNA barcoding has ...
Farhana Ramli Farah   +6 more
doaj   +1 more source

Oral Streptococcus salivarius Couples Neutrophil IRGM1 Signaling to NET Formation and Colorectal Cancer Metastasis

open access: yesAdvanced Science, EarlyView.
The oral bacterium Streptococcus salivarius promotes colorectal cancer metastasis by inducing neutrophil extracellular trap (NET) formation. Mechanistically, IRGM1–IQGAP1 interaction activates Wnt5a–PI3K/AKT signaling in neutrophils, driving NET‐mediated tumor progression.
Fengyi Liu   +13 more
wiley   +1 more source

Sphingomonas paucimobilis‐Driven Epithelial–Endothelial Transition in Adenomyosis Pathogenesis

open access: yesAdvanced Science, EarlyView.
This study identifies epithelial–endothelial transition (EET) as a novel adenomyosis pathogenic mechanism driven by Sphingomonas paucimobilis colonization. Systematic inhibitor experiments validate a TNFα→NF‐κB→MMP signaling cascade essential for EET. Critically, bacterial culture supernatant fails to induce pathological changes, demonstrating viable ...
Peigen Chen   +7 more
wiley   +1 more source

A DNA barcoding framework for taxonomic verification in the Darwin Tree of Life Project [version 1; peer review: 2 approved]

open access: yesWellcome Open Research
Biodiversity genomics research requires reliable organismal identification, which can be difficult based on morphology alone. DNA-based identification using DNA barcoding can provide confirmation of species identity and resolve taxonomic issues but is ...
Silvia Salatino   +40 more
doaj   +1 more source

A practical method for barcoding and size-trimming PCR templates for amplicon sequencing

open access: yesBioTechniques, 2016
Sample barcoding facilitates the analysis of tens or even hundreds of samples in a single next-generation sequencing (NGS) run, but more efficient methods are needed for high-throughput barcoding and size-trimming of long PCR products.
Anita Mäki   +2 more
doaj   +1 more source

Maternal Preconception Antibiotic Exposure Disrupts Microbial Succession: A Transgenerational Risk for Offspring Gut Mucosal Immaturity and Colitis Susceptibility

open access: yesAdvanced Science, EarlyView.
This study reveals that maternal antibiotic exposure prior to conception disrupts intergenerational gut microbial succession. By enhancing maternal‐offspring microbial transmission, altering microbial developmental trajectories and increasing selective pressures during community assembly, these disturbances lead to persistent gut mucosal immaturity and
Yuzhu Chen   +8 more
wiley   +1 more source

DNA Barcoding of Tabanids (Diptera: Tabanidae) from Veracruz, Mexico, with Notes on Morphology and Taxonomy

open access: yesTaxonomy
(1) Background: Tabanids are one of the most neglected and difficult-to-identify groups within the order Diptera despite their medical–veterinary importance.
Julia J. Alavez-Chávez   +7 more
doaj   +1 more source

Self‐Assembling Hybrid Hydrogel Reprograms the Stromal Vascular Fraction to Treat Osteoarthritis

open access: yesAdvanced Science, EarlyView.
This study presents a bioinspired injectable hydrogel that enhances the therapeutic potential of stem cell‐rich stromal vascular fraction for treating osteoarthritis. By reprogramming cell behavior through epigenetic modulation, the hydrogel promotes cartilage regeneration and reduces joint damage in a rat model, offering a promising new approach for ...
Waifang Hou   +23 more
wiley   +1 more source

DNA barcoding is currently unreliable for species identification in most crayfishes

open access: yesEcology and Evolution
DNA barcoding is commonly used for species identification. Despite this, there has not been a comprehensive assessment of the utility of DNA barcoding in crayfishes (Decapoda: Astacidea).
Patrick F. Allison Jr   +3 more
doaj   +1 more source

Hypofractionated Radiotherapy Induces ISG15+MHC‐I+ Neutrophils to Augment Anti‐Tumor Immunity and Prime Immune Checkpoint Blockade Responses in Rectal Cancer

open access: yesAdvanced Science, EarlyView.
ABSTRACT Our previous clinical trials had demonstrated that neoadjuvant hypofractionated radiotherapy (HFRT) combined with immunotherapy yields promising clinical outcomes in locally advanced rectal cancer (LARC). However, this combined modality benefits only a subset of patients, highlighting the need to uncover the mechanisms underlying how ...
Lichao Liu   +12 more
wiley   +1 more source

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