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DNA barcoding and the mediocrity of morphology [PDF]
Molecular Ecology Resources, 2009 AbstractA small but vocal community of critics has questioned the epistemological value of DNA barcoding by suggesting that either it ‘cannot work’ for the identification or discovery of species or that it ignores the ‘richness’ inherent in traditional approaches.
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2012
This chapter is an overview of the techniques for DNA barcoding of fishes from field collection to DNA sequence analysis. Recommendations for modifications of field protocols and best tissue sampling practices are made. A variety of DNA extraction protocols is provided, including high-throughput robot-assisted methods. A pair of well-tested forward and
Lee A, Weigt +3 more
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This chapter is an overview of the techniques for DNA barcoding of fishes from field collection to DNA sequence analysis. Recommendations for modifications of field protocols and best tissue sampling practices are made. A variety of DNA extraction protocols is provided, including high-throughput robot-assisted methods. A pair of well-tested forward and
Lee A, Weigt +3 more
openaire +2 more sources
2012
DNA barcoding refers to the technique of sequencing a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene, the "DNA barcode," from a taxonomically unknown specimen and performing comparisons with a reference library of barcodes of known species origin to establish a species-level identification. The library barcodes gain their
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DNA barcoding refers to the technique of sequencing a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene, the "DNA barcode," from a taxonomically unknown specimen and performing comparisons with a reference library of barcodes of known species origin to establish a species-level identification. The library barcodes gain their
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DNA barcoding of Canada’s skates
Molecular Ecology Resources, 2011AbstractDNA‐based identifications have been employed across broad taxonomic ranges and provide an especially useful tool in cases where external identification may be problematic. This study explored the utility of DNA barcoding in resolving skate species found in Atlantic Canadian waters.
M W, Coulson +5 more
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DNA Sequencing Technologies and DNA Barcoding
DNA barcodes are short, standardized DNA segments that geneticists can use to identify all living taxa. On the other hand, DNA barcoding identifies species by analyzing these specific regions against a DNA barcode reference library. In its initial years, DNA barcodes sequenced by Sanger's method were extensively used by taxonomists for the ...Anisha, David +2 more
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DNA Barcoding of Marine Metazoans
2016The accumulation of DNA barcode sequences will provide an increasingly useful and comprehensive library for species identification and discovery of marine metazoans. Here we present a summary of protocols designed to obtain DNA barcodes of marine metazoans from diverse phyla.
Dirk, Steinke +2 more
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DNA barcodes of Philippine accipitrids
Molecular Ecology Resources, 2010AbstractDNA barcoding is a molecular method that rapidly identifies an individual to a known taxon or its closest relative based on a 650‐bp fragment of the cytochrome c oxidase subunit I (COI). In this study, DNA barcodes of members of the family Accipitridae, including Haliastur indus (brahminy kite), Haliaeetus leucogaster (white‐bellied sea eagle),
Perry S, Ong +6 more
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DNA Barcoding Evolves into the Familiar
Conservation Biology, 2006First, there is nothing wrong with careful use of a DNA barcode to help identify an unknown specimen; indeed, in some cases, it might be the most effective way to find a name for many species. The caveat is that the unknown specimen needing identification is from a previously described (known to science) species.
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Generating 2D Barcode for DNA Barcode Sequences
DNA barcode sequence is a short DNA sequence representing a sample from a particular species. The commonly used DNA barcodes are at least 200 bps long. This large number of characters cannot be encoded in two-dimensional codes for sample recognition and tracking. In the present study, we described a method that can be used to compress the DNA sequencesRui, Liu +3 more
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DNA Barcodes: Methods and Protocols
2012DNA barcoding, a new method for the quick identification of any species based on extracting a DNA sequence from a tiny tissue sample of any organism, is now being applied to taxa across the tree of life. As a research tool for taxonomists, DNA barcoding assists in identification by expanding the ability to diagnose species by including all life history
W John, Kress, David L, Erickson
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