Results 21 to 30 of about 1,557,993 (359)

The Usefulness of Autoradiography for DNA Repair Proteins Activity Detection in the Cytoplasm towards Radiolabeled Oligonucleotides Containing 5′,8-Cyclo-2′-deoxyadenosine

Chemosensors, 2022
Autoradiography of 32P-radiolabeled oligonucleotides is one of the most precise detection methods of DNA repair processes. In this study, autoradiography allowed assessing the activity of proteins in the cytoplasm involved in DNA repair. The cytoplasm is
Karolina Boguszewska, Bolesław T. Karwowski, Julia Kaźmierczak-Barańska   +2 more
doaj   +1 more source

DNA damage and autophagy [PDF]

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2011
Both exogenous and endogenous agents are a threat to DNA integrity. Exogenous environmental agents such as ultraviolet (UV) and ionizing radiation, genotoxic chemicals and endogenous byproducts of metabolism including reactive oxygen species can cause alterations in DNA structure (DNA damage). Unrepaired DNA damage has been linked to a variety of human
Mihalis I. Panayiotidis, Humberto Rodriguez-Rocha, Rodrigo Franco, Aracely Garcia-Garcia   +3 more
openaire   +3 more sources

Elevated Level of DNA Damage and Impaired Repair of Oxidative DNA Damage in Patients with Recurrent Depressive Disorder [PDF]

, 2015
Background: Depressive disorder (DD), including recurrent DD (rDD), is a severe psychological disease, which affects a large percentage of the world population.
Bielecka-Kowalska, Anna, Czarny, Piotr, Gałecki, Piotr, Kacperska, Dagmara, Kawczyńska, Daria, Kwiatkowski, Dominik, Orzechowska, Agata, Szemraj, Janusz, Talarowska, Monika, Śliwiński, Tomasz   +9 more
core   +1 more source

Automation of the cytokinesis-block micronucleus cytome assay by laser scanning cytometry and its potential application in radiation biodosimetry

BioTechniques, 2014
Here we describe the adaptation of laser scanning cytometry (LSC) to measure micronuclei (MN) automatically in lymphocytes. MN frequencies were determined in irradiated human lymphocytes using the cytokinesis-block technique, and the results from LSC ...
Maxime François, Kevin Hochstenbach, Wayne Leifert, Michael Felix Fenech   +3 more
doaj   +1 more source

8-Oxo-7,8-Dihydro-2′-Deoxyguanosine (8-oxodG) and 8-Hydroxy-2′-Deoxyguanosine (8-OHdG) as a Potential Biomarker for Gestational Diabetes Mellitus (GDM) Development

Molecules, 2020
The growing clinical and epidemiological significance of gestational diabetes mellitus results from its constantly increasing worldwide prevalence, obesity, and overall unhealthy lifestyle among women of childbearing age. Oxidative stress seems to be the
Sandra K. Urbaniak, Karolina Boguszewska, Michał Szewczuk, Julia Kaźmierczak-Barańska, Bolesław T. Karwowski   +4 more
doaj   +1 more source

The Influence of 5′,8-Cyclo-2′-deoxypurines on the Mitochondrial Repair of Clustered DNA Damage in Xrs5 Cells: The Preliminary Study

Molecules, 2021
The 5′,8-cyclo-2′-deoxypurines (cdPus) affect the DNA structure. When these bulky structures are a part of clustered DNA lesions (CDL), they affect the repair of the other lesions within the cluster.
Karolina Boguszewska, Julia Kaźmierczak-Barańska, Bolesław T. Karwowski   +2 more
doaj   +1 more source

DNA Checkpoint and Repair Factors Are Nuclear Sensors for Intracellular Organelle Stresses-Inflammations and Cancers Can Have High Genomic Risks. [PDF]

, 2018
Under inflammatory conditions, inflammatory cells release reactive oxygen species (ROS) and reactive nitrogen species (RNS) which cause DNA damage. If not appropriately repaired, DNA damage leads to gene mutations and genomic instability.
Chen, Hongping, Choi, Eric T., Cueto, Ramon, Drosatos, Konstantinos, Fu, Hangfei, Nanayakkara, Gayani K, Park, Joon Y., Qin, Xuebin, Shao, Lijian, Shao, Ying, Sheng, Haitao, Sun, Jianxin, Sun, Yu, Wang, Hong, Wang, Luqiao, Wu, Na, Yang, Qian, Yang, William Y, Yang, Wuping, Yang, Xiaofeng, Zeng, Huihong, Zhu, Qingxian   +21 more
core   +2 more sources

The DNA damage response acts as a safeguardagainst harmful DNA–RNA hybrids ofdifferent origins [PDF]

, 2019
Despite playing physiological roles in specific situations, DNA–RNA hybrids threat genome integrity. To investigate how cells do counteract spontaneous DNA–RNA hybrids, here we screen an siRNA library covering 240 human DNA damage response (DDR) genes ...
Aguilera López, Andrés, Barroso Ceballos, Sonia Inés, García Rubio, María Luisa, Gómez González, Belén, Herrera Moyano, Emilia, Muñoz Sánchez, Sergio   +5 more
core   +1 more source

Novel Implications of DNA Damage Response in Drug Resistance of Malignant Cancers Obtained from the Functional Interaction between p53 Family and RUNX2

Biomolecules, 2015
During the lifespan of cells, their genomic DNA is continuously exposed to theendogenous and exogenous DNA insults. Thus, the appropriate cellular response to DNAdamage plays a pivotal role in maintaining genomic integrity and also acts as a ...
Toshinori Ozaki, Mizuyo Nakamura, Osamu Shimozato   +2 more
doaj   +1 more source

RanBP2-Mediated SUMOylation Promotes Human DNA Polymerase Lambda Nuclear Localization and DNA Repair [PDF]

, 2020
Cellular DNA is under constant attack by a wide variety of agents, both endogenous and exogenous. To counteract DNA damage, human cells have a large collection of DNA repair factors. Among them, DNA polymerase lambda (Polλ) stands out for its versatility,
Cortés Ledesma, Felipe, García Domínguez, Mario, Herrero Ruiz, Andrés M., Moreno Oñate, Marta, Ruiz Pérez, José Francisco   +4 more
core   +1 more source