Results 201 to 210 of about 939,296 (267)

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Cost-effective DNA extraction method optimized for high yield and long fragments from coastal sediments. [PDF]

PLoS One
Matthys L, Moungues EM, Genève A, Sanial V, Misson B, Navarro E.   +5 more
europepmc   +1 more source

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

FEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar   +11 more
wiley   +1 more source

Optimization of a magnetic bead-based DNA extraction method combined with 12S rRNA barcoding for species traceability in fish oil products. [PDF]

Food Chem (Oxf)
Zhao W, Jiang Q, Zhou X, Ye Z, Bao W, Wu J, Zhang Y.   +6 more
europepmc   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Rapid On-Site Detection of <i>Colletotrichum gloeosporioides</i> Using EASY DNA Extraction (EZ-D) Method Combined with RPA-CRISPR/Cas12a. [PDF]

Plants (Basel)
Yang C, Dai S, Wang B, Zang J, Kong Y, Chen C, Wang H, Dai T.   +7 more
europepmc   +1 more source

Robust, Open-Source and Automation-Friendly DNA Extraction Protocol for Hologenomic Research. [PDF]

Mol Ecol Resour
Lauritsen JG, Carøe C, Gaun N, Martin-Bideguren G, Leonard A, Eisenhofer R, Odriozola I, Gilbert MTP, Aizpurua O, Alberdi A, Pietroni C.   +10 more
europepmc   +1 more source

Methodology of DNA extraction and sequencing from living cardiomyocytes collected by catheter in humans. [PDF]

Genet Med Open
Ader F, Guilbeau-Frugier C, Lhuillier E, Martins F, Gonzalez AA, Rollin A, Beneyto M, Gales C, Pires F, Maoret JJ, Sénard JM, Timnou-Bekouti J, Villard E, Duboscq-Bidot L, Gandjbakhch E, Maury P.   +15 more
europepmc   +1 more source

Field-ready DNA extraction from scat using magnetic nanoparticles for non-invasive wildlife monitoring. [PDF]

Sci Rep
Dondi L, Chaudhari R, Schmitt N, Poissant J, Musiani M, Filipe CDM, Li Y.   +6 more
europepmc   +1 more source

The impact of DNA extraction methods on species quantification and apparent community composition of in vitro oral biofilms. [PDF]

FEMS Microbiol Lett
Reese JO, Castro Lund IM, Haugen HJ, Saragliadis A, Lyngstadaas SP, Linke D.   +5 more
europepmc   +1 more source