Results 261 to 270 of about 226,967 (304)
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Leukemia Research, 1985
Following partial purification on sucrose gradient and/or phosphocellulose chromatography, DNA ligase was tested in peripheral white blood and bone marrow cells of nearly 100 patients with various kinds of leukemias, mainly acute leukemias. Terminal deoxynucleotidyl transferase (TdT) was tested in parallel.
J C, David +9 more
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Following partial purification on sucrose gradient and/or phosphocellulose chromatography, DNA ligase was tested in peripheral white blood and bone marrow cells of nearly 100 patients with various kinds of leukemias, mainly acute leukemias. Terminal deoxynucleotidyl transferase (TdT) was tested in parallel.
J C, David +9 more
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Ligation of DNA with T4 DNA Ligase
2003Since they are involved in such important processes as DNA replication, DNA repair, and DNA recombination, DNA ligases can be found in all living cells. Two prokaryotic DNA ligases have become indispensible tools in the fields of in vitro DNA recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and
W, Gaastra, K, Hansen
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Prokaryotic DNA Ligases Unwind Superhelical DNA
Biochemical and Biophysical Research Communications, 1996We have studied the effect on DNA topology of binding of prokaryotic DNA ligases (T4 and E. coli) to superhelical or nicked circular DNA. Performing topoisomerase I-mediated relaxation in the presence of increasing amounts of T4 ligase led to a shift in the topoisomer distribution to increasingly more negative values.
M, Ivanchenko +2 more
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DNA Polymerases and DNA Ligases
2013DNA polymerase and DNA ligase are ubiquitous enzymes that synthesize complementary DNA strands according to the template DNA and ligate breaks in the backbone structure of DNA, respectively, in living organisms. Multiple enzymes have been identified from each organism, and the functional sharing of these enzymes has been investigated for both DNA ...
Sonoko Ishino, Yoshizumi Ishino
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DNA ligases and ligase-based technologies
Clinical and Applied Immunology Reviews, 2001Abstract DNA ligases catalyze the strand joining reaction at a nick junction. The requirement of base-pair complementarity at the nick junction has been explored for development of ligase-based technologies for mutation detection. In oligonucleotide ligation assay (OLA), two DNA probes complementary to the target sequence are joined by DNA ligase ...
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Eukaryotic DNA Ligases and DNA Repair
1998The correct joining of appropriate DNA ends is a fundamental step in many important reactions within the cell and a number of enzymes have been identified which perform these end-joining reactions. Some enzymes execute a ligation step as an intermediate of their overall reaction mechanism; DNA topoisomerases are a prime example of this type of activity
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Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation
Nucleic Acids Research, 2018Shih-Hsun Chen +2 more
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