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DNA ligases and ligase-based technologies

Clinical and Applied Immunology Reviews, 2001
Abstract DNA ligases catalyze the strand joining reaction at a nick junction. The requirement of base-pair complementarity at the nick junction has been explored for development of ligase-based technologies for mutation detection. In oligonucleotide ligation assay (OLA), two DNA probes complementary to the target sequence are joined by DNA ligase ...
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Eukaryotic DNA Ligases and DNA Repair

1998
The correct joining of appropriate DNA ends is a fundamental step in many important reactions within the cell and a number of enzymes have been identified which perform these end-joining reactions. Some enzymes execute a ligation step as an intermediate of their overall reaction mechanism; DNA topoisomerases are a prime example of this type of activity
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1 DNA Ligases

1982
Publisher Summary DNA ligases refer to enzymes that catalyze the formation of a phosphodiester linkage between DNA chains. DNA ligases are essential reagents in studies on nucleic acid structure and metabolism. Their value derives from the specificity of the reaction and their ability to join polynucleotide chains covalently.
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DNA Ligases

2005
A Montecucco, R Rossi
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Enzyme-regulated activation of DNAzyme: a novel strategy for a label-free colorimetric DNA ligase assay and ligase-based biosensing.