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Estimating belowground plant abundance with DNA metabarcoding
Molecular Ecology Resources, 2019AbstractMost work on plant community ecology has been performed above ground, neglecting the processes that occur in the soil. DNA metabarcoding, in which multiple species are computationally identified in bulk samples, can help to overcome the logistical limitations involved in sampling plant communities belowground.
Silvia Matesanz +10 more
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DNA metabarcoding data analysis
2018Abstract DNA metabarcoding generates huge amounts of data containing noise introduced by molecular methods. Chapter 8 “DNA metabarcoding data analysis” discusses the analytic steps and available software to curate and evaluate DNA metabarcoding data prior to final ecological analyses. It provides command lines to perform primary analyses
Pierre Taberlet +3 more
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DNA metabarcode choice and design
2018Abstract Chapter “DNA metabarcode choice and design” develops the properties of the ideal metabarcode in a given context, including conservation of the primer annealing regions and resolution power across the target taxonomic group of interest.
Pierre Taberlet +3 more
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Towards next‐generation biodiversity assessment using DNA metabarcoding
Molecular Ecology, 2012Virtually all empirical ecological studies require species identification during data collection. DNA metabarcoding refers to the automated identification of multiple species from a single bulk sample containing entire organisms or from a single environmental sample containing degraded DNA (soil, water, faeces, etc.).
Taberlet, Pierre +4 more
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Reconstructing marine plankton food web interactions using DNA metabarcoding
Molecular Ecology, 2020AbstractKnowledge of zooplankton in situ diet is critical for accurate assessment of marine ecosystem function and structure, but due to methodological constraints, there is still a limited understanding of ecological networks in marine ecosystems. Here, we used DNA‐metabarcoding to study trophic interactions, with the aim to unveil the natural diet of
Sara Zamora‐Terol +2 more
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Environmental DNA and RNA Metabarcoding
Environmental DNA (eDNA) and RNA (eRNA) technologies, when integrated with metabarcoding, have emerged as significant instruments in cancer research particularly in exposomics and environmental carcinogenesis. These approaches facilitate the identification and monitoring of genetic material from many environmental sources, yielding insights into the ...Odangowei Inetiminebi Ogidi +2 more
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A quantitative protocol for DNA metabarcoding of springtails (Collembola)
Genome, 2016We developed a novel protocol with superior quantitative analysis results for DNA metabarcoding of Collembola, a major soil microarthropod order. Degenerate PCR primers were designed for conserved regions in the mitochondrial cytochrome c oxidase subunit I (mtCOI) and 16S ribosomal RNA (mt16S) genes based on published collembolan mitogenomes. The best
Seikoh, Saitoh +8 more
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DNA Barcoding and Metabarcoding Protocols for Species Identification
The article presents the several steps to be performed on a plant, fungal, insect, or soil sample to obtain DNA sequences for DNA barcode analysis. The chapter begins with a description of sample preparation including procedures for cleaning and proceeds to DNA extraction with methods adapted for the specific type of sample. Next, DNA quantification isKyleen E, Elwick +2 more
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