Results 341 to 350 of about 410,424 (355)
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Current Genomics, 2003
Recent advances in DNA microarray technology have opened the door for large-scale gene expression screening, functional analysis and genomic profiling. Microarrays offer a new opportunity for genome-wide studies and are quickly revolutionizing biological analyses.
Daniel A. Peiffer+2 more
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Recent advances in DNA microarray technology have opened the door for large-scale gene expression screening, functional analysis and genomic profiling. Microarrays offer a new opportunity for genome-wide studies and are quickly revolutionizing biological analyses.
Daniel A. Peiffer+2 more
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DNA Microarray in Glycobiology
2009DNA microarray analysis enables us to provide a considerable amount of information on glyco-chain expression (Brazma et al. 2001; Ide et al. 2006). DNA microarray experiments are developed to obtain overall transcriptome of cells using DNAs spotted on a slide glass as probes and fluorescently labeled DNAs derived from mRNA samples.
Yasunori Kozutsumi+2 more
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DNA microarrays in prostate cancer
Current Urology Reports, 2002DNA microarray technology provides a means to examine large numbers of molecular changes related to a biological process in a high throughput manner. This review discusses plausible utilities of this technology in prostate cancer research, including definition of prostate cancer predisposition, global profiling of gene expression patterns associated ...
Ho, Shuk-Mei, Lau, Kin-Mang
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DNA microarrays for expression profiling
Current Opinion in Chemical Biology, 2001DNA microarrays enable the transcript levels of an entire genome to be measured simultaneously. Recent improvements in array manufacture, sample preparation, and data analysis are shifting emphasis from the technology itself to experimental design and the broader range of biological questions that can be addressed.
Daniel D. Shoemaker, Timothy P. Hughes
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DNA Microarray Technology in Dermatology
Seminars in Cutaneous Medicine and Surgery, 2008In recent years, DNA microarray technology has been used for the analysis of gene expression patterns in a variety of skin diseases, including malignant melanoma, psoriasis, lupus erythematosus, and systemic sclerosis. Many of the studies described herein confirmed earlier results on individual genes or functional groups of genes.
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2008
Although techniques such as RT-PCR and in situ hybridization (ISH) can give information about gene expression, they are limited in scope as typically one gene product is evaluated with each assay. The advent of transcriptional profiling using DNA microarray has revolutionized the field of molecular medicine as measurement of thousands of genes ...
Lyndsay Harris+2 more
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Although techniques such as RT-PCR and in situ hybridization (ISH) can give information about gene expression, they are limited in scope as typically one gene product is evaluated with each assay. The advent of transcriptional profiling using DNA microarray has revolutionized the field of molecular medicine as measurement of thousands of genes ...
Lyndsay Harris+2 more
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Indirect Labeling of DNA for Microarrays
Cold Spring Harbor Protocols, 2019As with RNA labeling protocols, the main difference between direct and indirect DNA labeling protocols is a trade-off of cost and time. Indirect labeling of DNA, described here, takes ∼2 h longer than direct labeling but is hundreds of dollars cheaper.
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DNA Microarrays: An Introduction to the Technology
2009DNA microarrays allow the comprehensive genetic analysis of an organism or a sample. They are based on probes, which are immobilized in an ordered two-dimensional pattern on substrates, such as nylon membranes or glass slides. Probes are either spotted cDNAs or oligonucleotides and are designed to be specific for an organism, a gene, a genetic variant (
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