Molecular dynamics study of the opening mechanism for DNA polymerase I. [PDF]
PLoS Computational Biology, 2014 During DNA replication, DNA polymerases follow an induced fit mechanism in order to rapidly distinguish between correct and incorrect dNTP substrates. The dynamics of this process are crucial to the overall effectiveness of catalysis.
Bill R Miller +2 more
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Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site. [PDF]
Biomed Res Int, 2016 The large fragment of DNA polymerase I from Geobacillus stearothermophilus GIM1.543 (Bst DNA polymerase) with 5′-3′ DNA polymerase activity while in absence of 5′-3′ exonuclease activity possesses high thermal stability and polymerase activity.
Ma Y +5 more
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Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1 [PDF]
MicrobiologyOpen, 2021 Several native and engineered heat‐stable DNA polymerases from a variety of sources are used as powerful tools in different molecular techniques, including polymerase chain reaction, medical diagnostics, DNA sequencing, biological diversity assessments ...
Ani Saghatelyan +3 more
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Crystal structures of DNA polymerase I capture novel intermediates in the DNA synthesis pathway [PDF]
eLife, 2018 High resolution crystal structures of DNA polymerase intermediates are needed to study the mechanism of DNA synthesis in cells. Here we report five crystal structures of DNA polymerase I that capture new conformations for the polymerase translocation and
Nicholas Chim +3 more
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Inhibitors against DNA Polymerase I Family of Enzymes: Novel Targets and Opportunities [PDF]
BiologyDNA polymerases replicate cellular genomes and/or participate in the maintenance of genome integrity. DNA polymerases sharing high sequence homology with E. coli DNA polymerase I (pol I) have been grouped in Family A.
Saathvik Kannan +5 more
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Single-molecule FRET reveals proofreading complexes in the large fragment of Bacillus stearothermophilus DNA polymerase I [PDF]
AIMS Biophysics, 2018 There is increasing interest in the use of DNA polymerases (DNA pols) in next-generation sequencing strategies. These methodologies typically rely on members of the A and B family of DNA polymerases that are classified as high-fidelity DNA polymerases ...
Thomas V. Christian +1 more
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Engineering a Thermostable Reverse Transcriptase for RT-PCR Through Rational Design of Pyrococcus furiosus DNA Polymerase [PDF]
BiomoleculesEngineering of a bifunctional enzyme that combines DNA-dependent DNA polymerase and reverse transcriptase (RT) activities is a highly promising biotechnological goal, as it would enable one-enzyme RT-PCR.
Aleksandra A. Kuznetsova +3 more
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Understanding the Fidelity and Specificity of DNA Polymerase I [PDF]
ACS OmegaBill R. Miller +7 more
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The discovery of the mechanisms of biological synthesis of nucleic acids: 1959 Nobel laureates S. Ochoa and A. Kornberg [PDF]
The Ukrainian Biochemical Journal, 2021 Alongside the chemical and physical research of nucleic acids in the 1940s-50s, the mechanisms of their biosynthesis were investigated. Thus, in 1959, Severo Ochoa and Arthur Kornberg were awarded the Nobel Prize in Physiology or Medicine for the ...
O. P. Matyshevska +2 more
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Cloning, Expression, and Characterization of Family A DNA Polymerase from Massilia aurea
Fermentation, 2023 Mau DNA polymerase is a family A DNA polymerase isolated from Massilia aurea. In this study, a recombinant plasmid, His6-tagged Mau-pET28c, was constructed.
Aleksandra A. Kuznetsova +3 more
doaj +1 more source