Results 101 to 110 of about 1,611,597 (332)

Aphidicolin inhibits DNA polymerase II ofEscherichia coli, an alpha-like DNA polymerase

Nucleic Acids Research, 1990
The polB gene of E. coli encodes DNA polymerase II. We have reported the cloning (1,2) and sequencing of the polB gene (3) (GenBank Accession number M35371). DNA polymerase II appears to be a non-essential function since the polB gene is missing from some widely used strains (3). Several sub-families of DNA polymerases have been identified (4). In this
H, Chen, C B, Lawrence, S K, Bryan, R E, Moses   +3 more
openaire   +3 more sources

Characterizing the salivary RNA landscape to identify potential diagnostic, prognostic, and follow‐up biomarkers for breast cancer

Molecular Oncology, EarlyView.
This study explores salivary RNA for breast cancer (BC) diagnosis, prognosis, and follow‐up. High‐throughput RNA sequencing identified distinct salivary RNA signatures, including novel transcripts, that differentiate BC from healthy controls, characterize histological and molecular subtypes, and indicate lymph node involvement.
Nicholas Rajan, Irina Primac, Emre Etlioglu, Laurens Debruyne, Ann Janssen, Magy Sallam, Kevin Tabury, Roel Quintens, Wiebren Tjalma, Mohammed Abderrafi Benotmane   +9 more
wiley   +1 more source

Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion

Nature Communications, 2013
The fidelity of DNA polymerases depends on conformational changes that promote the rejection of incorrect nucleotides before phosphoryl transfer. Here, we combine single-molecule FRET with the use of DNA polymerase I and various fidelity mutants to ...
J. Hohlbein, Louise Aigrain, Timothy D. Craggs, Oya Bermek, O. Potapova, Pouya Shoolizadeh, N. Grindley, C. M. Joyce, A. Kapanidis   +8 more
semanticscholar   +1 more source

Kinetic analysis of an efficient DNA-dependent TNA polymerase. [PDF]

, 2005
alpha-l-Threofuranosyl nucleoside triphosphates (tNTPs) are tetrafuranose nucleoside derivatives and potential progenitors of present-day beta-d-2'-deoxyribofuranosyl nucleoside triphosphates (dNTPs).
Chaput, John C, Horhota, Allen, Ichida, Justin K, McLaughlin, Larry W, Szostak, Jack W, Yu, Biao, Zou, Keyong   +6 more
core   +2 more sources

Bridging the gap: Multi‐stakeholder perspectives of molecular diagnostics in oncology

Molecular Oncology, EarlyView.
Although molecular diagnostics is transforming cancer care, implementing novel technologies remains challenging. This study identifies unmet needs and technology requirements through a two‐step stakeholder involvement. Liquid biopsies for monitoring applications and predictive biomarker testing emerge as key unmet needs. Technology requirements vary by
Jorine Arnouts, Senada Koljenović, Elise Daems, Karolien De Wael, Marc Peeters, Léon C. van Kempen, Greetje Vanhoutte, Karen Zwaenepoel, Timon Vandamme   +8 more
wiley   +1 more source

Unexpected role for Helicobacter pylori DNA polymerase I as a source of genetic variability.

PLoS Genetics, 2011
Helicobacter pylori, a human pathogen infecting about half of the world population, is characterised by its large intraspecies variability. Its genome plasticity has been invoked as the basis for its high adaptation capacity.
María-Victoria García-Ortíz, Stéphanie Marsin, Mercedes E Arana, Didier Gasparutto, Raphaël Guérois, Thomas A Kunkel, J Pablo Radicella   +6 more
doaj   +1 more source

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

Synthetic Nucleotides as Probes of DNA Polymerase Specificity

Journal of Nucleic Acids, 2012
The genetic code is continuously expanding with new nucleobases designed to suit specific research needs. These synthetic nucleotides are used to study DNA polymerase dynamics and specificity and may even inhibit DNA polymerase activity. The availability
Jason M. Walsh, Penny J. Beuning
doaj   +1 more source

Mutations that Separate the Functions of the Proofreading Subunit of the Escherichia coli Replicase

, 2015
The dnaQ gene of Escherichia coli encodes the Ɛ subunit of DNA polymerase III, which provides the 3\u27 - 5\u27 exonuclease proofreading activity of the replicative polymerase.
Kreuzer, Kenneth N., Whatley, Zakiya
core   +1 more source

A global function for transcription factors in assisting RNA polymerase II termination. [PDF]

, 2017
The role of transcription factors (TFs) on nucleosome positioning, RNA polymerase recruitment, and transcription initiation has been extensively characterized.
Chanfreau, Guillaume F, Roy, Kevin
core   +1 more source