Results 11 to 20 of about 1,611,597 (332)
Cells, 2022 DNA polymerase β plays a fundamental role in the life cycle of Trypanosoma cruzi since it participates in the kinetoplast DNA repair and replication. This enzyme can be found in two forms in cell extracts of T. cruzi epimastigotes form.
Edio Maldonado +5 more
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Conformational Dynamics of DNA Polymerases Revealed at the Single-Molecule Level
Frontiers in Molecular Biosciences, 2022 DNA polymerases are intrinsically dynamic macromolecular machines. The purpose of this review is to describe the single-molecule Förster resonance energy transfer (smFRET) methods that are used to probe the conformational dynamics of DNA polymerases ...
David P. Millar
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Microorganisms, 2021 DNA polymerase B1 (PolB1) is a member of the B-family DNA polymerase family and is a replicative DNA polymerase in Crenarchaea. PolB1 is responsible for the DNA replication of both the leading and lagging strands in the thermophilic crenarchaeon ...
Hiroka Miyabayashi +2 more
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Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. [PDF]
Molecular and Cellular Biology, 1989 We have determined the fidelity of DNA synthesis by DNA polymerase I (yPol I) from Saccharomyces cerevisiae. To determine whether subunits other than the polymerase catalytic subunit influence fidelity, we measured the accuracy of yPol I purified by conventional procedures, which yields DNA polymerase with a partially proteolyzed catalytic subunit and ...
T A, Kunkel +4 more
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Biosensors and Bioelectronics: X, 2023 Herein, we developed a novel method for identifying protein-small molecule interactions (PSMIs) based on the switching of DNA polymerase activity. This strategy employs the small molecule-linked DNA probe (SMP) that could bind to and disrupt the DNA ...
Junhyeok Yoon +4 more
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Conditions for Using DNA Polymerase I as an RNA-Dependent DNA Polymerase [PDF]
Proceedings of the National Academy of Sciences, 1974 Conditions are described for using Escherichia coli DNA polymerase I for synthesizing complementary DNA copies of natural RNA molecules, which are suitable for use in hybridization experiments. The molar ratio of enzyme to template is critical; below a certain level, synthesis is not observed.
S C, Gulati, D L, Kacian, S, Spiegelman
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Opposed Steric Constraints in Human DNA Polymerase β and E. coli DNA Polymerase I [PDF]
Journal of the American Chemical Society, 2008 DNA polymerase selectivity is crucial for the survival of any living species, yet varies significantly among different DNA polymerases. Errors within DNA polymerase-catalyzed DNA synthesis result from the insertion of noncanonical nucleotides and extension of misaligned DNA substrates.
Di Pasquale, Francesca +5 more
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The DNA polymerases of Drosophila melanogaster [PDF]
Fly, 2020 DNA synthesis during replication or repair is a fundamental cellular process that is catalyzed by a set of evolutionary conserved polymerases. Despite a large body of research, the DNA polymerases of Drosophila melanogaster have not yet been systematically reviewed, leading to inconsistencies in their nomenclature, shortcomings in their functional ...
Marygold, Steven J +9 more
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Bst polymerase — a humble relative of Taq polymerase
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair.
Igor Oscorbin, Maxim Filipenko
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Single-molecule view of coordination in a multi-functional DNA polymerase
eLife, 2021 Replication and repair of genomic DNA requires the actions of multiple enzymatic functions that must be coordinated in order to ensure efficient and accurate product formation.
Raymond F Pauszek III +3 more
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