A novel plasmid-based co-tethered transcription platform for high yield, high purity mRNA synthesis. [PDF]
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Genetic Evidence of Active Circulation and Evolution of Diverse Penguin Siadenoviruses in Antarctica Based on Partial DNA Sequences. [PDF]
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Kinetic mechanism of DNA polymerase I (Klenow).
Biochemistry, 1987The minimal kinetic scheme for DNA polymerization catalyzed by the Klenow fragment of DNA polymerase I (KF) from Escherichia coli has been determined with short DNA oligomers of defined sequence. A key feature of this scheme is a minimal two-step sequence that interconverts the ternary KF.DNAn.dNTP and KF.DNAn+1.PPi complexes.
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DNA polymerase III is an enzyme activity in eukaryotic cells which under certain conditions shows strong preference for polyadenylic acid as template when primed by oligodeoxythymidylate. Its first complete separation from other DNA polymerases in human lymphoblasts is reported.
Brian J. Lewis +3 more
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Domain of E. coli DNA polymerase I showing sequence homology to T7 DNA polymerase
Nature, 1985Escherichia coli contains three DNA polymerases that differ in their size, ability to interact with accessory proteins and biological function. Monomeric DNA polymerase I (Pol I) has a relative molecular mass (Mr) of 103,000 (103K) and is involved primarily in the repair of damaged DNA and the processing of Okazaki fragments; polymerase II is of Mr ...
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