Results 291 to 300 of about 1,611,597 (332)

Structure of DNA polymerase I Klenow fragment bound to duplex DNA

Science, 1993
Klenow fragment of Escherichia coli DNA polymerase I, which was cocrystallized with duplex DNA, positioned 11 base pairs of DNA in a groove that lies at right angles to the cleft that contains the polymerase active site and is adjacent to the 3' to 5 ...
L. Beese, V. Derbyshire, T. Steitz
semanticscholar   +1 more source

Cellular role of DNA polymerase I

Journal of Basic Microbiology, 1990
AbstractEscherichia coli possesses three well‐established DNA polymerases, I, II and III. DNA polymerase I (Pol 1) is the main repair polymerase in E. coli and also has a minor but important role in chromosomal replication. A major advantage of Pol I as an experimental system is its simplicity: unlike other replication enzymes, it is active as a single
Savić, D.J., Janković, M., Kostić, Tatjana   +2 more
openaire   +3 more sources

DNA AmplificationIn VitroUsing T4 DNA Polymerase

DNA, 1988
We have evaluated in vitro DNA amplification by polymerase chain reaction using either T4 DNA polymerase or Klenow fragment of Escherichia coli DNA polymerase I. Both polymerases under optimal salt conditions permit efficient amplification of exon 3 of the hypoxanthine guanine phosphoribosyltransferase (HPRT) gene from human genomic DNA and from ...
P, Keohavong, A G, Kat, N F, Cariello, W G, Thilly   +3 more
openaire   +2 more sources

Action of DNA polymerase I on γ-irradiated DNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
Abstract 1. The action of DNA polymerase I (EC 2.7.7.7) on DNA containing 3′-hydroxyl terminated single-strand breaks and on DNA γ-irradiated in dilute solution was studied. 2. The incorporation of deoxyribonucleotides into 3′-hydroxyl terminated DNA by DNA polymerase I increases with increasing concentration of 3′-hydroxyl end groups leading to a ...
Landbeck, L., Hagen, U.
openaire   +2 more sources

DNA polymerase I revisited

Medical Hypotheses, 1976
Summary A model for DNA replicating machinery is proposed which proposes that DNA polymerase I has the potential to synthesize the 3′→5′ strand of DNA and also that nucleotidediphosphokinases are closely associated with the polymerase. Some experiments are suggested that will answer the questions raised.
openaire   +2 more sources

DNA polymerase I: essential replication enzyme.

Science (New York, N.Y.), 1976
I. Lehman, D. Uyemura
semanticscholar   +4 more sources

Mutability of DNA polymerase I: Implications for the creation of mutant DNA polymerases

DNA Repair, 2005
DNA polymerases of the Family A catalyze the addition of deoxynucleotides to a primer with high efficiency, processivity, and selectivity-properties that are critical to their function both in nature and in the laboratory. These polymerases tolerate many amino acid substitutions, even in regions that are evolutionarily conserved.
Ern, Loh, Lawrence A, Loeb
openaire   +2 more sources

DNA Polymerase I from Acinetobacter calcoaceticus: Enzymatic Properties

European Journal of Biochemistry, 1974
Certain enzymatic properties of homogenous DNA polymerase I from Acinetobacter calcoaceticus have been investigated. The enzyme requires an initiator DNA for normal DNA repair synthesis. It is, however, also capable of de novo synthesis in the presence of dATP and dTTP or dGTP and dCTP.
I F, Nes, J R, Lillehaug, K, Kleppe
openaire   +2 more sources

Human DNA polymerase 3 (R-DNA polymerase): distinction from DNA polymerase I and reverse transcriptase.

Science (New York, N.Y.), 1974
DNA polymerase III is an enzyme activity in eukaryotic cells which under certain conditions shows strong preference for polyadenylic acid as template when primed by oligodeoxythymidylate. Its first complete separation from other DNA polymerases in human lymphoblasts is reported.
B J, Lewis, J W, Abrell, R G, Smith, R C, Gallo   +3 more
openaire   +1 more source

DNA polymerase action on benzo[a]pyrene—DNA adducts

Carcinogenesis, 1992
A 16mer oligonucleotide containing a single guanine residue at nucleotide 13 from the 3' end was treated with the (+)-enantiomer of the 7,8-dihydrodiol 9,10-epoxide of benzo[a]pyrene (B[a]P). Oligonucleotides containing either an adduct in which the epoxide ring was opened trans or cis by the amino group of the guanine residue were separated by ...
A M, Hruszkewycz, K A, Canella, K, Peltonen, L, Kotrappa, A, Dipple   +4 more
openaire   +2 more sources