Structures of LIG1 that engage with mutagenic mismatches inserted by polβ in base excision repair
Nature Communications, 2022 Ligase I seals two ends of DNA during DNA repair and replication. Here — solving structures of Ligase I with imperfect DNA harboring mismatches incorporated by DNA polymerase — the authors reveal how the repair proteins maintain fidelity at final ...
Qun Tang +3 more
doaj +1 more source
Pif1 Helicase Lengthens Some Okazaki Fragment Flaps Necessitating Dna2 Nuclease/Helicase Action in the Two-nuclease Processing Pathway [PDF]
, 2009 We have developed a system to reconstitute all of the proposed steps of Okazaki fragment processing using purified yeast proteins and model substrates.
Bambara, Robert A. +3 more
core +2 more sources
Engineered DNA Polymerase Improves PCR Results for Plastid DNA
Applications in Plant Sciences, 2013 Premise of the study: Secondary metabolites often inhibit PCR and sequencing reactions in extractions from plant material, especially from silica-dried and herbarium material.
Melanie Schori +3 more
doaj +1 more source
I-PfoP3I: a novel nicking HNH homing endonuclease encoded in the group I intron of the DNA polymerase gene in Phormidium foveolarum phage Pf-WMP3. [PDF]
PLoS ONE, 2012 Homing endonucleases encoded in a group I self-splicing intron in a protein-coding gene in cyanophage genomes have not been reported, apart from some free-standing homing edonucleases.
Shuanglei Kong +4 more
doaj +1 more source
Role for RNA: DNA hybrids in origin-independent replication priming in a eukaryotic system [PDF]
, 2015 DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuring complete genome duplication within a single S-phase. A key feature of replication origins is their ability to control the onset of DNA synthesis mediated by ...
Aguilera López, Andrés +3 more
core +1 more source
The DNA polymerases ofLeishmania mexicana [PDF]
FEMS Microbiology Letters, 1992 Two previously isolated DNA polymerases from the parasitic protozoan Leishmania mexicana were further characterized by exposure to inhibitors of mammalian DNA polymerases. DNA polymerase A, a high molecular mass enzyme, and DNA polymerase B, a beta-like DNA polymerase were compared to each other and to their mammalian counterparts regarding pH optimum,
L L, Nolan, J H, Rivera
openaire +2 more sources
Indonesian Journal of Biotechnology, 2010 Thermostable DNA polymerase has an important role for amplifying small amount of DNA through polymerase chain reaction (PCR). Thermophillic bacteria Brevibacillus sp. was isolated from Sikidang Crater, Dieng Plateu, Central Java.
Lucia Dhiantika Witasari +3 more
doaj +1 more source
Inventory and Evolution of Mitochondrion-localized Family A DNA Polymerases in Euglenozoa
Pathogens, 2020 The order Trypanosomatida has been well studied due to its pathogenicity and the unique biology of the mitochondrion. In Trypanosoma brucei, four DNA polymerases, namely PolIA, PolIB, PolIC, and PolID, related to bacterial DNA polymerase I (PolI), were ...
Ryo Harada +10 more
doaj +1 more source
Structure of an Intranucleosomal DNA Loop That Senses DNA Damage during Transcription
Cells, 2022 Transcription through chromatin by RNA polymerase II (Pol II) is accompanied by the formation of small intranucleosomal DNA loops containing the enzyme (i-loops) that are involved in survival of core histones on the DNA and arrest of Pol II during the ...
Nadezhda S. Gerasimova +7 more
doaj +1 more source
Novel Antibiotics Targeting Bacterial Replicative DNA Polymerases
Antibiotics, 2020 Multidrug resistance is a worldwide problem that is an increasing threat to global health. Therefore, the development of new antibiotics that inhibit novel targets is of great urgency.
Joana A. Santos, Meindert H. Lamers
doaj +1 more source