Results 41 to 50 of about 1,611,597 (332)
Single-molecule microscopy reveals new insights into nucleotide selection by DNA polymerase I. [PDF]
, 2012 The mechanism by which DNA polymerases achieve their extraordinary accuracy has been intensely studied because of the linkage between this process and mutagenesis and carcinogenesis. Here, we have used single-molecule fluorescence microscopy to study the
Markiewicz, RP +3 more
core +1 more source
Comprehensive analysis of the chromatin landscape in Drosophila melanogaster. [PDF]
, 2010 Chromatin is composed of DNA and a variety of modified histones and non-histone proteins, which have an impact on cell differentiation, gene regulation and other key cellular processes.
Alekseyenko, Artyom A +29 more
core +4 more sources
Biomolecules, 2020 Among the three domains of life, the process of homologous recombination (HR) plays a central role in the repair of double-strand DNA breaks and the restart of stalled replication forks. Curiously, main protein actors involved in the HR process appear to
Gaëlle Hogrel +7 more
doaj +1 more source
DNA polymerase I and DNA primase complex in yeast.
Journal of Biological Chemistry, 1984 Chromatographic analysis of poly(dT) replication activity in fresh yeast extracts showed that the activities required co-fractionate with the yeast DNA polymerase I. Since poly(dT) replication requires both a primase and a DNA polymerase, the results of the fractionation studies suggest that these two enzymes might exist as a complex in the yeast ...
P. Plevani +3 more
openaire +4 more sources
The polymerase domain of Streptococcus pneumoniae DNA polymerase I [PDF]
European Journal of Biochemistry, 1991 The 3′‐terminal two‐thirds of the Streptococcus pneumoniae polA gene was cloned in an Escherichia coli genefusion vector with inducible expression. The resulting recombinant plasmid (pSM10) directs the hyperproduction of a polypeptide of 70.6 kDa corresponding to the C‐terminal fragment of pneumococcal DNA polymerase I.
M E, Pons +3 more
openaire +2 more sources
MoleculesDNA polymerases are important enzymes that synthesize DNA molecules and therefore are critical to various scientific fields as essential components of in vitro DNA synthesis reactions, including PCR.
Zhongchen Li +8 more
doaj +1 more source
Reconstitution of recombination-associated DNA synthesis with human proteins. [PDF]
, 2013 The repair of DNA breaks by homologous recombination is a high-fidelity process, necessary for the maintenance of genome integrity. Thus, DNA synthesis associated with recombinational repair must be largely error-free.
Grossi, Sara M +4 more
core +1 more source
DNA polymerase beta can substitute for DNA polymerase I in the initiation of plasmid DNA replication [PDF]
Journal of Bacteriology, 1995 We previously demonstrated that mammalian DNA polymerase beta can substitute for DNA polymerase I of Escherichia coli in DNA replication and in base excision repair. We have now obtained genetic evidence suggesting that DNA polymerase beta can substitute for E. coli DNA polymerase I in the initiation of replication of a plasmid containing a pMB1 origin
J B, Sweasy, M, Chen, L A, Loeb
openaire +2 more sources
Journal of Molecular Biology, 1998 A two-metal-ion catalytic mechanism has previously been proposed for several phosphoryl-transfer enzymes. In order to extend the structural basis of this mechanism, crystal structures of three single-stranded DNA substrates bound to the 3'-5 ...
C. Brautigam, T. Steitz
semanticscholar +1 more source
Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity [PDF]
, 2015 CRISPR-Cas is a prokaryotic immune system built from capture and integration of invader DNA into CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) loci, termed ‘Adaptation’, which is dependent on Cas1 and Cas2 proteins.
Edward L Bolt +5 more
core +2 more sources