Molecular Oncology, EarlyView.Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka +10 more
wiley +1 more source
Detection of single-base mutations by reaction of DNA heteroduplexes with a water-soluble carbodiimide followed by primer extension: application to products from the polymerase chain reaction [PDF]
, 1990 Arupa Ganguly, Darwin J. Prockop
openalex +1 more source
Molecular Oncology, EarlyView.TGF‐β has a complex role in cancer, exhibiting both tumor‐suppressive and tumor‐promoting properties. Using a series of differentiated tumoroids, derived from different stages and mutational background of colorectal cancer patients, we replicate this duality of TGF‐β in vitro. Notably, the atypical but highly aggressive KRASQ22K mutation rendered early‐
Theresia Mair +17 more
wiley +1 more source
Specific Nucleic Acid Detection Using a Nanoparticle Hybridization Assay [PDF]
arXivSimple methods to detect biomolecules including specific nucleic acid sequences have received renewed attention since the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) virus pandemic. Notably, biomolecule detection that uses some form of signal amplification will have some form of amplification-related error, which in the polymerase ...
arxiv
PRIMER ACTIVITY OF THYMUS DNA FRACTIONATED BY ECTEOLA COLUMN CHROMATOGRAPHY
, 1963 Wesley D. Skidmore +2 more
openalex +2 more sources
DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting [PDF]
, 1994 Igor Brikun +2 more
openalex +1 more source
The Effect of Mismatch Primers on the Efficiency of Amplification in Quantitative Polymerase Chain Reactions [PDF]
, 2018 Polymerase chain reaction (PCR) is a method used in many research protocols to amplify a small amount of a short segment of DNA to millions of copies. PCR is used for many taxonomic studies, as well as for some medical diagnostic procedures. Through PCR,
Dawkins, Molly C
core +1 more source
Molecular Oncology, EarlyView.There is an unmet need in metastatic breast cancer patients to monitor therapy response in real time. In this study, we show how a noninvasive and affordable strategy based on sequencing of plasma samples with longitudinal tracking of tumour fraction paired with a statistical model provides valuable information on treatment response in advance of the ...
Emma J. Beddowes +20 more
wiley +1 more source
A PCR method for introducing mutations into cloned DNA by joining an internal primer to a tagged flanking primer [PDF]
, 1992 Jörg Stappert +2 more
openalex +1 more source
In silico estimation of annealing specificity of query searches in DNA databases [PDF]
, 2005 We consider DNA implementations of databases for digital signals with retrieval and mining capabilities. Digital signals are encoded in DNA sequences and retrieved through annealing between query DNA primers and data carrying DNA target sequences.
Hatzimanikati, Vassily +2 more
core