Results 221 to 230 of about 4,042,809 (333)

AFLP: a new technique for DNA fingerprinting.

Nucleic Acids Research, 1995
P. Vos, R. Hogers, M. Bleeker, M. Reijans, Theo van de Lee, Miranda Hornes, A. Friters, J. Pot, J. Paleman, M. Kuiper, M. Zabeau   +10 more
semanticscholar   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

A Simple Restriction Fragment Length Polymorphism-Based Method for Multiplex Testing of Thrombosis Risk Factors FV Leiden and <i>F2</i> G20210A with Highly Sensitive Contamination Detection. [PDF]

Int J Mol Sci
de Mazancourt P, Grey S, Alabre E, Keita M, Rabès JP.   +4 more
europepmc   +1 more source

cis -Acting Sequences 5E, M, and 3E Interact To Contribute to Primer Translocation and Circularization during Reverse Transcription of Avian Hepadnavirus DNA

, 2002
Karlyn Mueller-Hill, Daniel D. Loeb
openalex   +2 more sources

16S ribosomal DNA amplification for phylogenetic study

Journal of Bacteriology, 1991
W. Weisburg, S. M. Barns, D. Pelletier, S. Goodison   +3 more
semanticscholar   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Enhanced blood parasite species identification using V4-V9 18S rDNA barcoding by universal primers on a nanopore platform. [PDF]

Sci Rep
Sugi T, Reteng P, Gaithuma AK, Kawai N, Namangala B, Mutengo MM, Ishiguro N, Hayashida K, Yamagishi J.   +8 more
europepmc   +1 more source

Authenticationof the Botanical Origin of Honey: In Silico Assessmentof Primers for DNA Metabarcoding

Lucia Ranieri (21475533), Lucilia Lorusso (21475536), Anna Mottola (6592808), Chiara Intermite (21475539), Roberta Piredda (4849423), Angela Di Pinto (6592805)   +5 more
openalex   +1 more source

Loop-mediated isothermal amplification of DNA.

Nucleic Acids Research, 2000
T. Notomi, H. Okayama, Harumi Otawara-shi Masubuchi, Toshihiro Yonekawa, Keiko Watanabe, N. Amino, T. Hase   +6 more
semanticscholar   +1 more source

Naked cuticle is essential for Drosophila wing development beyond Wingless signaling

FEBS Open Bio, EarlyView.
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley   +1 more source