Results 61 to 70 of about 763,201 (203)
Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning
BMC Biotechnology, 2019 Background Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length limit of current DNA padlock probe
Syukri Shukor +4 more
doaj +1 more source
Real-Time PCR Detection of Bacillus anthracis by Lambda_Ba03 Prophage Genes
Проблемы особо опасных инфекций, 2022 The aim of the study was to develop a set of primers and fluorescent probes for the detection of two chromosomal targets of Bacillus anthracis using real-time PCR based on the lambda_Ba03 prophage genes.Materials and methods.
A. S. Nizkorodova +10 more
doaj +1 more source
Direct probing: covalent attachment of probe DNA to double-stranded target DNA [PDF]
Nucleic Acids Research, 1998 We report a novel procedure, which can be applied to probing of specific DNA, for covalently attaching probe DNA to complementary sequences in double-stranded target DNA. Employing hairpin-like oligonucleotide probes in combination with successive use of recA protein and DNA ligase, probes can be attached directly to target DNA molecules without ...
J, Fujiwara, M, Oishi
openaire +2 more sources
BMC BiologyBackground Probe-based liquid-phase hybridization capture is a powerful and commonly used approach for next-generation sequencing (NGS) of mitochondrial DNA (mtDNA).
Tianlei Sun +8 more
doaj +1 more source
BMC Infectious Diseases, 2003 Background We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
Miller Donna L +7 more
doaj +1 more source
G3: Genes, Genomes, Genetics, 2021 Rapeseed is a globally cultivated commercial crop, primarily grown for its oil. High-density single nucleotide polymorphism (SNP) arrays are widely used as a standard genotyping tool for rapeseed research, including for gene mapping, genome-wide ...
Qing Xiao +14 more
doaj +1 more source
The Scientific World Journal, 2003 Analyses of complex microbial communities are becoming increasingly important. Bottlenecks in these analyses, however, are the tools to actually describe the biodiversity.
Knut Rudi +3 more
doaj +1 more source
BiosensorsProtein-based microarrays are important tools for high-throughput medical diagnostics, offering versatile platforms for multiplex immunodetection. However, challenges arise in protein microarrays due to the heterogeneous nature of proteins and, thus ...
Sylwia Karoń +2 more
doaj +1 more source
Genome-wide analysis of DNA methylation in buccal cells: a study of monozygotic twins and mQTLs
Epigenetics & Chromatin, 2018 Background DNA methylation arrays are widely used in epigenome-wide association studies and methylation quantitative trait locus (mQTL) studies. Here, we performed the first genome-wide analysis of monozygotic (MZ) twin correlations and mQTLs on data ...
Jenny van Dongen +13 more
doaj +1 more source
Beating the reaction limits of biosensor sensitivity with dynamic tracking of single binding events [PDF]
, 2018 The clinical need for ultrasensitive molecular analysis has motivated the development of several endpoint-assay technologies capable of single-molecule readout.
Sevenler, Derin +2 more
core +2 more sources