Results 61 to 70 of about 789,440 (249)
Hibridación in situ fluorescente (FISH) [PDF]
, 2016 Background: At the end of 80s, cloning technologies with the increase of the antibodies’ sensibility made easier the development of technologies based on Fluorescence in situ Hibridation (FISH).
Aguilar, Ruben, Montoro, Carolina
core
Intra- and Interspecies Analyses of the Carcinoembryonic Antigen (CEA) Gene Family Reveal Independent Evolution in Primates and Rodents [PDF]
, 1989 Various rodent and primate DNAs exhibit a stronger intra- than interspecies cross-hybridization with probes derived from the N-terminal domain exons of human and rat carcinoembryonic antigen (CEA)-like genes.
Rudert, Fritz +2 more
core +1 more source
Frontiers in Genome Editing, 2022 An extensive arsenal of biosensing tools has been developed based on the clustered regularly interspaced short palindromic repeat (CRISPR) platform, including those that detect specific DNA sequences both in vitro and in live cells.
Nicholas G. Heath +11 more
doaj +1 more source
Direct probing: covalent attachment of probe DNA to double-stranded target DNA [PDF]
Nucleic Acids Research, 1998 We report a novel procedure, which can be applied to probing of specific DNA, for covalently attaching probe DNA to complementary sequences in double-stranded target DNA. Employing hairpin-like oligonucleotide probes in combination with successive use of recA protein and DNA ligase, probes can be attached directly to target DNA molecules without ...
J, Fujiwara, M, Oishi
openaire +2 more sources
BMC BiologyBackground Probe-based liquid-phase hybridization capture is a powerful and commonly used approach for next-generation sequencing (NGS) of mitochondrial DNA (mtDNA).
Tianlei Sun +8 more
doaj +1 more source
Real-Time PCR Detection of Bacillus anthracis by Lambda_Ba03 Prophage Genes
Проблемы особо опасных инфекций, 2022 The aim of the study was to develop a set of primers and fluorescent probes for the detection of two chromosomal targets of Bacillus anthracis using real-time PCR based on the lambda_Ba03 prophage genes.Materials and methods.
A. S. Nizkorodova +10 more
doaj +1 more source
The Scientific World Journal, 2003 Analyses of complex microbial communities are becoming increasingly important. Bottlenecks in these analyses, however, are the tools to actually describe the biodiversity.
Knut Rudi +3 more
doaj +1 more source
Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning
BMC Biotechnology, 2019 Background Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length limit of current DNA padlock probe
Syukri Shukor +4 more
doaj +1 more source
, 2014 Citation: 'DNA probe' in the IUPAC Compendium of Chemical Terminology, 3rd ed.; International Union of Pure and Applied Chemistry; 2006. Online version 3.0.1, 2019. 10.1351/goldbook.D01826 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms.
openaire +1 more source
Probing the Mechanical Unzipping of DNA [PDF]
Physical Review Letters, 2006 A study of the micromechanical unzipping of DNA in the framework of the Peyrard-Bishop-Dauxois model is presented. We introduce a Monte Carlo technique that allows accurate determination of the dependence of the unzipping forces on unzipping speed and temperature. Our findings agree quantitatively with experimental results for homogeneous DNA, and for $
Voulgarakis, N. K. +3 more
openaire +3 more sources