Results 281 to 290 of about 185,882 (314)

DNA Cleavage by the Type IC Restriction-Modification EnzymeEcoR124II

Journal of Molecular Biology, 1996
Type I restriction-modification systems bind to non-palindromic, bipartite recognition sequences. Although these enzymes methylate specific adenine residues within their recognition sequences, they cut DNA at sites up to several thousand base-pairs away.
J, Dreier, M P, MacWilliams, T A, Bickle
openaire   +2 more sources

Real-time observation of DNA translocation by the type I restriction modification enzyme EcoR124I

Nature Structural & Molecular Biology, 2004
Type I restriction enzymes bind sequence-specifically to unmodified DNA and subsequently pull the adjacent DNA toward themselves. Cleavage then occurs remotely from the recognition site. The mechanism by which these members of the superfamily 2 (SF2) of helicases translocate DNA is largely unknown.
Ralf, Seidel, John, van Noort, Carsten, van der Scheer, Joost G P, Bloom, Nynke H, Dekker, Christina F, Dutta, Alex, Blundell, Terence, Robinson, Keith, Firman, Cees, Dekker   +9 more
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Identification of a gene encoding a DNA invertase-like enzyme adjacent to the PaeR7I restriction-modification system

Gene, 1995
A gene encoding a DNA invertase-like enzyme was identified adjacent to the PaeR7I restriction-modification system (R-M), and was named paeR7IN (N for iNvertase). Sequence analysis revealed that this gene has the same polarity as the PaeR7IRM operon, and would encode a polypeptide of 21,506 Da.
R, Vaisvila, G, Vilkaitis, A, Janulaitis
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The DNA recognition subunit of the type IB restriction-modification enzyme EcoAI tolerates circular permutions of its polypeptide chain

Journal of Molecular Biology, 1998
The DNA specificity subunit (HsdS) of type I restriction-modification enzymes is composed of two independent target recognition domains and several regions whose amino acid sequence is conserved within an enzyme family. The conserved regions participate in intersubunit interactions with two modification subunits (HsdM) and two restriction subunits ...
Pavel Janščák, Thomas A. Bickle
openalex   +3 more sources

Characterization of anEcoR 124I restriction-modification enzyme produced from a deleted form of the DNA-binding subunit, which results in a novel DNA specificity

Folia Microbiologica, 2003
We purified and characterized both the methyltransferase and the endonuclease containing the HsdS delta 50 subunit (type I restriction endonucleases are composed of three subunits--HsdR required for restriction, HsdM required for methylation and HsdS responsible for DNA recognition) produced from the deletion mutation hsdS delta 50 of the type IC R-M ...
Abadjieva, A, Scarlett, G, Janscak, Pavel, Dutta, C F, Firman, K   +4 more
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DNA cleavage by type III restriction-modification enzyme EcoP15I is independent of spacer distance between two head to head oriented recognition sites.

Journal of molecular biology, 2001
The type III restriction-modification enzyme EcoP15I requires the interaction of two unmethylated, inversely oriented recognition sites 5'-CAGCAG in head to head configuration to allow an efficient DNA cleavage. It has been hypothesized that two convergent DNA-translocating enzyme-substrate complexes interact to form the active cleavage complex and ...
M, Mücke, S, Reich, E, Möncke-Buchner, M, Reuter, D H, Krüger   +4 more
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The in Vitro Assembly of the EcoKI Type I DNA Restriction/Modification Enzyme and Its in Vivo Implications

Biochemistry, 1997
Type I DNA restriction/modification enzymes protect the bacterial cell from viral infection by cleaving foreign DNA which lacks N6-adenine methylation within a target sequence and maintaining the methylation of the targets on the host chromosome. It has been noted that the genes specifying type I systems can be transferred to a new host lacking the ...
D T, Dryden, L P, Cooper, P H, Thorpe, O, Byron   +3 more
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Remodeling of nucleosome by a DNA translocating bacterial restriction-modification enzyme

AbstractA eukaryotic cell has specialized ATP-dependent chromatin remodelers, such as SWI/SNF, to unfurl DNA from nucleosome for functional processing. The ATPase that powers the movement of the chromatin remodeler on the DNA (translocation) is evolutionarily related to those powering the translocation of the functionally distinct bacterial restriction-
Sujata S. Gaiwala Sharma, Mahesh Kumar Chand, Ishtiyaq Ahmed, Nibrasul Haque, Kayarat Saikrishnan   +4 more
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The assembly of the EcoKI type I DNA restriction/modification enzyme and its interaction with DNA

Biochemical Society Transactions, 1999
D T, Dryden, G D, Davies, I, Martin, L M, Powell, N E, Murray, D J, Ellis, T, Berge, J M, Edwardson, R M, Henderson   +8 more
openaire   +3 more sources

Carbohydrate-active enzymes (CAZymes) in the gut microbiome

Nature Reviews Microbiology, 2022
Jacob F Wardman, Peter Rahfeld, Stephen G Withers   +2 more
exaly