Results 51 to 60 of about 4,499,395 (250)

DNA methylation assessed by SMRT sequencing is linked to mutations in Neisseria meningitidis isolates [PDF]

, 2015
The Gram-negative bacterium Neisseria meningitidis features extensive genetic variability. To present, proposed virulence genotypes are also detected in isolates from asymptomatic carriers, indicating more complex mechanisms underlying variable ...
Gerd Pluschke, Christoph D. Schmid (72046), Schmid, Christoph D., Mane, Shrikant, Korlach, Jonas, Tyson A. Clark (105783), Katharina Röltgen, Wang, Guilin, Araceli Lamelas, Jonas Korlach (105788), Shrikant Mane, Araceli Lamelas (128475), Christoph D Schmid, Mohamad R Abdul Sater, Clark, Tyson A., Röltgen, Katharina, Gerd Pluschke (7855), Katharina Röltgen (120045), Guilin Wang, Sater, Mohamad R. Abdul, Tyson A Clark, Mohamad R. Abdul Sater (836669), Jonas Korlach, Guilin Wang (37620), Shrikant Mane (106631), Lamelas, Araceli, Pluschke, Gerd   +26 more
core   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

An intragenic distribution bias of DNA uptake sequences in Pasteurellaceae and Neisseriae [PDF]

, 2008
Most sequenced strains from Pasteurellaceae and Neisseriae contain hundreds to thousands of uptake sequence (US) motifs in their genome, which are associated with natural competence for DNA uptake. The mechanism of their recognition is still unclear, and
van Passel Mark WJ, Passel, M.W.J., van, Mark WJ van Passel, van Passel, M.W.J.   +3 more
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

The minor groove-binding agent ELB-21 forms multiple interstrand and intrastrand covalent cross-links with duplex DNA and displays potent bactericidal activity against methicillin-resistant Staphylococcus aureus [PDF]

, 2011
Objectives The antistaphylococcal pyrrolobenzodiazepine dimer ELB-21 forms multiple adducts with duplex DNA through covalent interactions with appropriately spaced guanine residues; it is now known to form interstrand and intrastrand adducts with ...
Rahman, Khondaker; id_orcid, Thurston, DE, Taylor, PW, Feuerbaum, EA, Helena Rosado, David E. Thurston, Rahman, KM, Peter W. Taylor, Rosado, H, Hinds, J, Khondaker M. Rahman, Jason Hinds, Eva-Anne Feuerbaum   +12 more
core   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species

Memorias do Instituto Oswaldo Cruz, 2013
As the distribution of Candida species and their susceptibility to antifungal agents have changed, a new means of accurately and rapidly identifying these species is necessary for the successful early resolution of infection and the subsequent reduction ...
Constanza Giselle Taverna, María Eugenia Bosco-Borgeat, Omar Alejandro Murisengo, Graciela Davel, Mariana Côrtes Boité, Elisa Cupolillo, Cristina Elena Canteros   +6 more
doaj   +1 more source

Hot-spot consensus of fluoroquinolone-mediated DNA cleavage by gram-negative and gram-positive type II DNA topoisomerases [PDF]

, 2007
Bacterial DNA gyrase and topoisomerase IV are selective targets of fluoroquinolones. Topoisomerase IV versus gyrase and Gram-positive versus Gram-negative behavior was studied based on the different recognition of DNA sequences by topoisomerase ...
GIARETTA G, COMUZZI V, Palumbo, M, Leo, E, Mitchenall, LA, Richter, SN, Maxwell, A, Fisher, LM, RICHTER, SARA, Giaretta, G, LEO E, MAXWELL A, Comuzzi, V, FISHER LM, MITCHENALL LA, PALUMBO, MANLIO   +15 more
core   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

A novel constraint for thermodynamically designing DNA sequences. [PDF]

PLoS ONE, 2013
Biotechnological and biomolecular advances have introduced novel uses for DNA such as DNA computing, storage, and encryption. For these applications, DNA sequence design requires maximal desired (and minimal undesired) hybridizations, which are the ...
Qiang Zhang, Bin Wang, Xiaopeng Wei, Changjun Zhou   +3 more
doaj   +1 more source