PIMS sequencing extension:a laboratory information management system for DNA sequencing facilities [PDF]
Background: Facilities that provide a service for DNA sequencing typically support large numbers of users and experiment types. The cost of services is often reduced by the use of liquid handling robots but the efficiency of such facilities is hampered ...
McPherson, MJ +18 more
core +1 more source
Introduction The introduction of pneumococcal conjugate vaccines (PCV) into the national immunization programs (NIPs) has significantly reduced the number of pneumococcal infections.
Weronika Puzia +9 more
doaj +1 more source
The microbiome of the human lower airways: a next generation sequencing perspective
For a long time, the human lower airways were considered a sterile environment where the presence of microorganisms, typically revealed by culturing, was interpreted as an abnormal health state.
Velma T.E. Aho +5 more
doaj +1 more source
From cheek swabs to consensus sequences : an A to Z protocol for high-throughput DNA sequencing of complete human mitochondrial genomes [PDF]
Background: Next-generation DNA sequencing (NGS) technologies have made huge impacts in many fields of biological research, but especially in evolutionary biology. One area where NGS has shown potential is for high-throughput sequencing of complete mtDNA
Matisoo-Smith, Elizabeth A +83 more
core +1 more source
Case Report of COVID-19 after Full Vaccination: Viral Loads and Anti-SARS-CoV-2 Antibodies
The introduction of effective vaccines against SARS-CoV-2 is expected to prevent COVID-19. However, sporadic cases of infection in vaccinated persons have been reported. We describe a case of a double-dose vaccinated woman with COVID-19.
Magdalena Komiazyk +5 more
doaj +1 more source
Automatic Preparation of DNA Templates for Sequencing on the ABI Catalyst Robotic Workstation [PDF]
The need for automation of the various steps in DNA sequencing increases as the number of applications and genomic sequencing applications grow. It is therefore necessary to design robotic workstations for template preparation and sequencing reactions in
Holmberg, A., +5 more
core +1 more source
The sequence of sequencers: The history of sequencing DNA
Determining the order of nucleic acid residues in biological samples is an integral component of a wide variety of research applications. Over the last fifty years large numbers of researchers have applied themselves to the production of techniques and technologies to facilitate this feat, sequencing DNA and RNA molecules. This time-scale has witnessed
Heather, JM, Chain, B
openaire +3 more sources
DNA methylation assessed by SMRT sequencing is linked to mutations in Neisseria meningitidis isolates [PDF]
The Gram-negative bacterium Neisseria meningitidis features extensive genetic variability. To present, proposed virulence genotypes are also detected in isolates from asymptomatic carriers, indicating more complex mechanisms underlying variable ...
Gerd Pluschke +26 more
core +1 more source
Illuminating Choices for Library Prep: A Comparison of Library Preparation Methods for Whole Genome Sequencing of Cryptococcus neoformans Using Illumina HiSeq. [PDF]
The industry of next-generation sequencing is constantly evolving, with novel library preparation methods and new sequencing machines being released by the major sequencing technology companies annually.
Mathew A Beale +14 more
core +1 more source
Background The complex cell wall structure of algae often precludes efficient extraction of their genetic material. The purpose of this study was to design a next-generation sequencing-suitable DNA isolation method for unicellular, achlorophyllous, yeast-
Tomasz Jagielski +5 more
doaj +1 more source

