Results 11 to 20 of about 1,871,498 (248)

Length-independent DNA packing into nanopore zero-mode waveguides for low-input DNA sequencing [PDF]

open access: yes, 2017
Compared with conventional methods, single-molecule real-time (SMRT) DNA sequencing exhibits longer read lengths than conventional methods, less GC bias, and the ability to read DNA base modifications.
Henley, Robert Y.   +4 more
core   +1 more source

Highly Resistant Serotype 19A Streptococcus pneumoniae of the GPSC1/CC320 Clone from Invasive Infections in Poland Prior to Antipneumococcal Vaccination of Children

open access: yesInfectious Diseases and Therapy, 2023
Introduction The introduction of pneumococcal conjugate vaccines (PCV) into the national immunization programs (NIPs) has significantly reduced the number of pneumococcal infections.
Weronika Puzia   +9 more
doaj   +1 more source

The microbiome of the human lower airways: a next generation sequencing perspective

open access: yesWorld Allergy Organization Journal, 2015
For a long time, the human lower airways were considered a sterile environment where the presence of microorganisms, typically revealed by culturing, was interpreted as an abnormal health state.
Velma T.E. Aho   +5 more
doaj   +1 more source

Case Report of COVID-19 after Full Vaccination: Viral Loads and Anti-SARS-CoV-2 Antibodies

open access: yesDiagnostics, 2021
The introduction of effective vaccines against SARS-CoV-2 is expected to prevent COVID-19. However, sporadic cases of infection in vaccinated persons have been reported. We describe a case of a double-dose vaccinated woman with COVID-19.
Magdalena Komiazyk   +5 more
doaj   +1 more source

Exploring genome wide bisulfite sequencing for DNA methylation analysis in livestock: a technical assessment [PDF]

open access: yes, 2014
peer-reviewedRecent advances made in “omics” technologies are contributing to a revolution in livestock selection and breeding practices. Epigenetic mechanisms, including DNA methylation are important determinants for the control of gene expression in ...
Couldrey, Christine, Doherty, Rachael
core   +2 more sources

16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform [PDF]

open access: yes, 2016
peer-reviewedBackground Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the
Claesson, Marcus J.   +4 more
core   +2 more sources

An optimized method for high quality DNA extraction from microalga Prototheca wickerhamii for genome sequencing

open access: yesPlant Methods, 2017
Background The complex cell wall structure of algae often precludes efficient extraction of their genetic material. The purpose of this study was to design a next-generation sequencing-suitable DNA isolation method for unicellular, achlorophyllous, yeast-
Tomasz Jagielski   +5 more
doaj   +1 more source

Identification of single nucleotides in MoS2 nanopores [PDF]

open access: yes, 2015
Ultrathin membranes have drawn much attention due to their unprecedented spatial resolution for DNA nanopore sequencing. However, the high translocation velocity (3000-50000 nt/ms) of DNA molecules moving across such membranes limits their usability.
Bulushev, Roman D.   +6 more
core   +2 more sources

Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi

open access: yesBMC Genomics, 2019
Background The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer of plant cell wall polysaccharides, modifier of softwood and hardwood lignin, and is able to produce ethanol from various waste ...
Mari Mäkinen   +9 more
doaj   +1 more source

A first insight into the genome of Prototheca wickerhamii, a major causative agent of human protothecosis

open access: yesBMC Genomics, 2021
Background Colourless microalgae of the Prototheca genus are the only known plants that have consistently been implicated in a range of clinically relevant opportunistic infections in both animals and humans.
Zofia Bakuła   +7 more
doaj   +1 more source

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