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Batch Optimization for DNA Synthesis [PDF]
Large pools of synthetic DNA molecules have been recently used to reliably store significant volumes of digital data. While DNA as a storage medium has enormous potential because of its high storage density, its practical use is currently severely limited because of the high cost and low throughput of available DNA synthesis technologies.
Konstantin Makarychev+3 more
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DNA Synthesis is the polymeric linkage of deoxyribose moieties (with adenine, guanine, cytosine, or thymine side chains) by phosphate groups attached to their 5-prime and 3-prime sugar hydroxyls.
semanticscholar +1 more source
Background Colourless microalgae of the Prototheca genus are the only known plants that have consistently been implicated in a range of clinically relevant opportunistic infections in both animals and humans.
Zofia Bakuła+7 more
doaj +1 more source
Photon-directed multiplexed enzymatic DNA synthesis for molecular digital data storage
New storage technologies are needed to keep up with the global demands of data generation. DNA is an ideal storage medium due to its stability, information density and ease-of-readout with advanced sequencing techniques.
Howon Lee+6 more
semanticscholar +1 more source
Translesion DNA Synthesis [PDF]
All living organisms are continually exposed to agents that damage their DNA, which threatens the integrity of their genome. As a consequence, cells are equipped with a plethora of DNA repair enzymes to remove the damaged DNA. Unfortunately, situations nevertheless arise where lesions persist, and these lesions block the progression of the
John P. McDonald+2 more
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Assessment of enzymatically synthesized DNA for gene assembly
Phosphoramidite chemical DNA synthesis technology is utilized for creating de novo ssDNA building blocks and is widely used by commercial vendors. Recent advances in enzymatic DNA synthesis (EDS), including engineered enzymes and reversibly terminated ...
Brooke L. Simmons+4 more
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DNA-templated synthesis optimization [PDF]
In chemistry, synthesis is the process in which a target compound is produced in a step-wise manner from given base compounds. A recent, promising approach for carrying out these reactions is DNA-templated synthesis, since, as opposed to more traditional methods, this approach leads to a much higher effective molarity and makes much desired one-pot ...
Bjarke N. Hansen+3 more
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The DNA polymerases of Drosophila melanogaster
DNA synthesis during replication or repair is a fundamental cellular process that is catalyzed by a set of evolutionary conserved polymerases.
Steven J. Marygold+9 more
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An important step in preparation of cells for cell therapy and tissue engineering is the cultivation of cells in vitro. The aim of this work is to study the effect of human apolipoprotein A-I (apoA-I) on the functional activity of cultured bone marrow ...
A. Yu. Gorodetskaya+4 more
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Background The complex cell wall structure of algae often precludes efficient extraction of their genetic material. The purpose of this study was to design a next-generation sequencing-suitable DNA isolation method for unicellular, achlorophyllous, yeast-
Tomasz Jagielski+5 more
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