Results 121 to 130 of about 1,995,166 (228)

An Optimized Freeze‐Dry Multimodal Workflow for Sequential Micro‐CT Imaging, Histology, and Molecular Profiling: A Use Case in Human Liver Fibrosis

Advanced Science, EarlyView.
Optimized freeze‐drying and eosin staining deliver artifact‐free micro‐CT images of liver tissue at near‐histological detail, then hand the same specimen back for sectioning, staining, and sequencing. Fibrotic volume distinguishes normal liver from cirrhosis, while preserved RNA reveals inflammatory gene upregulation—one biopsy, three dimensions, and a
Kristijan Skok, Laura Činč Ćurić, Jernej Vajda, Silvia Schauer, Martin Wagner, Rudolf Stauber, Carolin Lackner, Karl Kashofer, Markus Plass, Giovanny Rodriguez Blanco, Ian R. Wanless, Uroš Maver, Katrin Panzitt, Boštjan Vihar   +13 more
wiley   +1 more source

Top1-PARP1 association and beyond: from DNA topology to break repair. [PDF]

NAR Cancer, 2021
Chowdhuri SP, Das BB.
europepmc   +1 more source

The Topology of Branching Universes [PDF]

, 2005
The purpose of this paper is to survey the possible topologies of branching space-times, and, in particular, to refute the popular notion in the literature that a branching space-time requires a non-Hausdorff ...
McCabe, Gordon, Gordon Mccabe
core  

Polymer Concepts in Cellular Function

Advanced Science, EarlyView.
Miao Yu, Shikha Dhiman, Dorothee Dormann, Frauke Gräter, Katja Luck, Friederike Schmid, Andreas Walther, Edward A. Lemke   +7 more
wiley   +1 more source

Single‐Molecule Imaging of Endogenous RNA Using a Miniaturized and Circularly Permuted CRISPR‐Cas13 Degron‐Masking System CDegSR

Advanced Science, EarlyView.
CDegSR, a new tool for imaging RNA at single molecule level in living cells was developed by researchers. Background noise was eliminated by protein engineering and making it degrade when not bound to its target. This allowed individual RNA molecules to be clearly imaged and tracked.
Shipeng Shao, Hongchen Zhang
wiley   +1 more source

Mutational synergy during leukemia induction remodels chromatin accessibility, histone modifications and three-dimensional DNA topology to alter gene expression. [PDF]

Nat Genet, 2021
Yun H, Narayan N, Vohra S, Giotopoulos G, Mupo A, Madrigal P, Sasca D, Lara-Astiaso D, Horton SJ, Agrawal-Singh S, Meduri E, Basheer F, Marando L, Gozdecka M, Dovey OM, Castillo-Venzor A, Wang X, Gallipoli P, Müller-Tidow C, Osborne CS, Vassiliou GS, Huntly BJP.   +21 more
europepmc   +1 more source

In vivo DNA topology and conformations of chromatin

, 2017
Trabajo presentado en el EMBO Workshop: DNA topoisomerases and DNA topology, celebrado en Les Diablerets (Suiza), del 17 al 21 de septiembre de 2017In contrast to our approximated view of the topology and conformations of naked DNA molecules in vitro ...
Dyson, Sílvia, Valdés, Antonio, Martínez-García, Belén, Joshi, Ricky S., Segura, Joana, Roca, Joaquim   +5 more
core  

Bone Marrow Macrophage STING Signaling is a Direct Nexus Linking Marrow Adipose Expansion to NAD+ Depletion and Osteogenic Decline in Skeletal Aging

Advanced Science, EarlyView.
This study reveals that during skeletal aging, bone marrow adipocyte‐derived sDPP4 primes STING signaling in macrophages. In combination with mitochondrial DNA, this signaling activates CD38 and depletes local NAD+/NMN, thereby impairing osteogenesis.
Zhichao Li, Kuanhui Gao, Mengjie Wang, Songlin Liang, Dandan Li, Guoqing Tan, Haipeng Xue, Yujie Liu, Peng Zhang, Zhanwang Xu, Wenxiang Cheng, Yongjun Wang, Nianhu Li   +12 more
wiley   +1 more source

Distinct Cleavage Trajectories Mediated by DNA and RNA Substrate in Argonaute From Methanocaldococcus Fervens

Advanced Science, EarlyView.
This study characterizes MfAgo, an enzyme exhibiting highly efficient DNA‐guided DNA and RNA cleavage capability. Precise RNA cleavage strictly requires dimerization to stabilize the PIWI‐loop region, while its robust DNA cleavage functions in a dimerization‐independent manner.
Fuyong Huang, Taiyu Chen, Meng Fang, Qinmiao Duanmu, Kangle Mu, Yuqi Bi, Siwei Xu, Xin Tao, Jiening Wang, Shan Wu   +9 more
wiley   +1 more source

Sex‐ and Time‐Resolved Physiological and Gut Ecosystem Responses to a Food‐Grade Microbiome‐Targeted Intervention in Collegiate Endurance Athletes

Advanced Science, EarlyView.
In collegiate endurance athletes, a 6‐week randomized CPYE intervention yielded time‐ and sex‐dependent shifts in field‐based cardiorespiratory, metabolic, and redox indices, accompanied by changes in gut multi‐omics profiles. An exploratory extension during intensified training generates hypotheses warranting future validation.
Meng Zhang, Le Ge, Kaiyang Liu, Yanrong Liu, Guolin Zhao, Lai‐Yu Kwok, Zheng Sun, Zhihong Sun, Wenyi Zhang   +8 more
wiley   +1 more source