Results 91 to 100 of about 1,893,739 (263)

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Role of DNA Topology in Biological Machines and Evolutionarily Related Biological Functions: Chemical Chirality in Site-Specific DNA Recombination

, 2019
Difference topology: The DNA path within a high-order DNA-protein assembly can be revealed from the topology of DNA knots and links formed by the action of Flp or Cre site-specific recombinase on target sites placed close to (and flanking) the assembly ...
Jayaram, Makkuni
core   +1 more source

Role of the central cations in the mechanical unfolding of DNA and RNA G-quadruplexes. [PDF]

, 2015
Cations are known to mediate diverse interactions in nucleic acids duplexes but they are critical in the arrangement of four-stranded structures. Here, we use all-atom molecular dynamics simulations with explicit solvent to analyse the mechanical ...
Bergues-Pupo, AE, Fiasconaro A., Ana Elisa Bergues-pupo, Bergues-Pupo, A.E., Arias-Gonzalez, J. R., Morón, M.C., Fiasconaro, A., Bergues Pupo, Ana Elisa, Arias-Gonzalez, J. Ricardo, Arias-Gonzalez, J., Fiasconaro, Alessandro, Bergues-Pupo, A., Falo, Fernando, Morón, MC, Fiasconaro, A, Bergues-Pupo A.E., Morón, M. Carmen, Falo, F, J. Ricardo Arias-gonzalez, Ro Fiasconaro, Morón M.C., Falo, F., Arias-Gonzalez, JR, Falo F., Arias-Gonzalez J.R., O Falo   +25 more
core   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

DNA Topology and Global Architecture of Point Centromeres

Cell Reports, 2015
DNA is wrapped in a left-handed fashion around histone octasomes containing the centromeric histone H3 variant CENP-A. However, DNA topology studies have suggested that DNA is wrapped in a right-handed manner around the CENP-A nucleosome that occupies ...
Ofelia Díaz-Ingelmo, Belén Martínez-García, Joana Segura, Antonio Valdés, Joaquim Roca   +4 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Bacterial DNA topology and infectious disease [PDF]

Nucleic Acids Research, 2008
The gram-negative bacterium Escherichia coli and its close relative Salmonella enterica have made important contributions historically to our understanding of how bacteria control DNA supercoiling and of how supercoiling influences gene expression and vice versa.
Dorman, Charles J., Corcoran, Colin P.
openaire   +4 more sources

Retroviral intasomes search for a target DNA by 1D diffusion which rarely results in integration

Nature Communications, 2016
Retroviral Integration into the host DNA is a rare event with evidence suggesting that it relies on DNA topology and sequence. Here the authors present single molecule evidence that shows the retroviral integration machinery vigorously scans the target ...
Nathan D. Jones, Miguel A. Lopez Jr, Jeungphill Hanne, Mitchell B. Peake, Jong-Bong Lee, Richard Fishel, Kristine E. Yoder   +6 more
doaj   +1 more source

FEDS: a Novel Fluorescence-Based High-Throughput Method for Measuring DNA Supercoiling In Vivo

mBio, 2020
DNA supercoiling (DS) is essential for life because it controls critical processes, including transcription, replication, and recombination. Current methods to measure DNA supercoiling in vivo are laborious and unable to examine single cells.
Alexandre Duprey, Eduardo A. Groisman
doaj   +1 more source

Cellular structures in Topology [PDF]

, 1990
This book describes the construction and the properties of CW-complexes. These spaces are important because firstly they are the correct framework for homotopy theory, and secondly most spaces that arise in pure mathematics are of this type.
Renzo Piccinini, Rudolf Fritsch, Fritsch, Rudolf, Piccinini, Renzo A., Rudolf Fritsch, et al.   +4 more
core   +1 more source