Results 71 to 80 of about 1,995,166 (228)
DNA Topology and Global Architecture of Point Centromeres
Cell Reports, 2015 DNA is wrapped in a left-handed fashion around histone octasomes containing the centromeric histone H3 variant CENP-A. However, DNA topology studies have suggested that DNA is wrapped in a right-handed manner around the CENP-A nucleosome that occupies ...
Ofelia Díaz-Ingelmo +4 more
doaj +1 more source
FEDS: a Novel Fluorescence-Based High-Throughput Method for Measuring DNA Supercoiling
mBio, 2020 DNA supercoiling (DS) is essential for life because it controls critical processes, including transcription, replication, and recombination. Current methods to measure DNA supercoiling in vivo are laborious and unable to examine single cells.
Alexandre Duprey, Eduardo A. Groisman
doaj +1 more source
A minimal cellulosome‐like system in Cellulosilyticum lentocellum
FEBS Open Bio, EarlyView.Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan +2 more
wiley +1 more source
A localized transition in the size variation of circular DNA in nanofluidic slitlike confinement
AIP Advances, 2013 We report strong evidence for a localized transition in the size variation of circular DNA between strong and moderate regimes of slitlike confinement. A novel and rigorous statistical analysis was applied to our recent experimental measurements of DNA ...
Elizabeth A. Strychalski +2 more
doaj +1 more source
Quinolones: Mechanism, Lethality and Their Contributions to Antibiotic Resistance
Molecules, 2020 Fluoroquinolones (FQs) are arguably among the most successful antibiotics of recent times. They have enjoyed over 30 years of clinical usage and become essential tools in the armoury of clinical treatments. FQs target the bacterial enzymes DNA gyrase and
Natassja G. Bush +3 more
doaj +1 more source
FEBS Open Bio, EarlyView.Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata +3 more
wiley +1 more source
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Variants in SLC6A1, encoding the GABA transporter 1 (GAT‐1), cause epilepsy, autism spectrum disorder, and developmental delay via loss of GABA uptake, impaired trafficking, and ER retention. We previously found that 4‐Phenylbutyrate (PBA), an FDA‐approved drug, restores GABA uptake and reduces seizures in SLC6A1‐related disorders ...
Melissa B. DeLeeuw +5 more
wiley +1 more source
DNA topology as a factor affecting transcription in bacteria [PDF]
DNA topology plays a key role in regulation of gene expression by affecting interactions between DNA and RNA polymerase and other regulatory proteins. In this work, I review the process of transcription and selected mechanisms of its regulation, focusing
Hladíková, Kristýna
core +1 more source
Frontiers in Genetics, 2019 DNA methylation plays a critical role in tumorigenesis through regulating oncogene activation and tumor suppressor gene silencing. Although extensively analyzed, the implication of DNA methylation in gene regulatory network is less characterized.
Jun Wu +9 more
doaj +1 more source
Advanced Functional Materials, EarlyView.DNA strands are employed both as dynamic linkers and nanoscale templates for the integration of Ag2S nanoparticles on MoS2, which in turn imparted photothermal responsiveness; this feature permits the selective cargo (fluorophore, quantum dots or an enzyme) release from the MoS2 surface in response to local heat induced by light irradiation.
Kai Chen +3 more
wiley +1 more source