Results 111 to 120 of about 94,155 (309)

Heptageniidae (Ephemeroptera) of Wisconsin [PDF]

, 2017
(excerpt) Heptageniidae are one of the most abundant and widespread components of Wisconsin\u27s aquatic insect fauna. In almost any stream with a firm substrate and free of gross pollution, the flattened nymphs can be found hiding in crevasses and under
Flowers, R. Wills, Hilsenhoff, William L
core   +3 more sources

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

A preliminary study of the effect of closed incision management with negative pressure wound therapy over high-risk incisions [PDF]

, 2015
Background Certain postoperative wounds are recognised to be associated with more complications than others and may be termed high-risk. Wound healing can be particularly challenging following high-energy trauma where wound necrosis and infection ...
A Colli, A Koski, A Li, A Wackenfors, A Wackenfors, AE Guille, BJ Stanley, CJ Jordan, D Herscovici Jr, DA Mendonca, David M. R. Sajik, DR Holt, DV Kipaldi, E Krug, EJ Harvey, EJ Timmenga, GI Taylor, GK Berry, GW Stanway, J Bertran, J Compston, J Kanis, JC Murrell, JP Stannard, JP Stannard, JS Weese, JT Thompson, JW Fox, Karen L. Perry, KV Lambert, L Holton, LJ Owen, Lynda Rutherford, M Demaria, M Pachowsky, MF Fay, Mieghan Bruce, MJ Allen, MJ Morykwas, MJ Morywkas, MJ Morywkas, MW Bohling, MW Bohling, N Fitzpatrick, NA Abidi, PC Bristow, PE Banwell, PE Banwell, PM Taylor, R Ben Amotz, R Burkhardt, R Petzina, RP Wilkes, S Karlakki, S Lindstedt, SA Robertson, SP Roch, SZ Chen, TJ Smith, WT DeCarbo   +59 more
core   +3 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Evidence of Morphological and Morphometric Differences in the Sella Turcica of Pteronotus mesoamericanus and P. mexicanus

Animals
Morphological modifications are a potential mechanism for functional species and phylogenetic diversification. The sella turcica in mammals is a structure associated with the basisphenoid bone and serves as the receptacle for the pituitary gland; however,
M. A. Peralta-Pérez, M. Briones-Salas
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

An unidentified harvestman Leiobunum sp. alarmingly invading Europe (Arachnida: Opiliones) [PDF]

, 2010
Etwa seit dem Jahr 2000 breitet sich eine bisher nicht identifizierte Art der Gattung Leiobunum C. L. Koch, 1839 schnell in Mitteleuropa aus. Nachweise ab dem Jahr 2004 sind aus den Niederlanden (wahrscheinlich dem Land des ersten Auftretens in Europa ...
Martens, Jochen, Schönhofer, Axel Ludwig, Wijnhoven, Hay   +2 more
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source