Results 101 to 110 of about 594,798 (294)

Dapagliflozin prevents methylglyoxal‐induced retinal cell death in ARPE‐19 cells

FEBS Open Bio, EarlyView.
Diabetic macular oedema is a diabetes complication of the eye, which may lead to permanent blindness. ARPE‐19 are human retinal cells used to study retinal diseases and potential therapeutics. Methylglyoxal is a compound increased in uncontrolled diabetes due to elevated blood glucose.
Naina Trivedi, Zainab Quraishi, Shima Khezri Azizi Far, Madaleine Ward, Aidan Conway, Reem Abdulredha, Eman Mshari, Yousif A. Shamsaldeen   +7 more
wiley   +1 more source

Dynamic analysis on a buried horizontal double-layer SF-type liquidstorage tank by finite element method

Electronic Journal of Structural Engineering, 2018
To study the dynamic responses of a buried horizontal double-layer SF-type liquid-storage tank considering liquid-solid interaction, a three-dimensional finite element model of the double-layer buried horizontal liquid-storage tank is established, and ...
X. Cheng, L. Hu, W. Zhang, D. Yu
doaj   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Study on vibro-acoustic transmission characteristics of ship's double-layer acoustic bridge

Zhongguo Jianchuan Yanjiu
ObjectivesThe inter-ship structure between double-layer shells is an important bridge for the transmission of acoustic vibration from pressure shell to light shell.
Zihao LIU, Fulin ZHOU, Bing LI, Zilong PENG, Jun FAN   +4 more
doaj   +1 more source

Spontaneous Coherence and Collective Modes in Double-Layer Quantum Dot Systems

, 1996
We study the ground state and the collective excitations of parabolically-confined double-layer quantum dot systems in a strong magnetic field. We identify parameter regimes where electrons form maximum density droplet states, quantum-dot analogs of the ...
A. H. MacDonald, A. H. MacDonald, A. H. MacDonald, A. H. MacDonald, A. Maksym, A.-P. Jauho, A.H. MacDonald, A.H. MacDonald, B.I. Halperin, C. de Chamon, D.B. Chklovskii, D.B. Mast, D.H. Lee, E. Dagotto, G.S. Solomon, H. A. Fertig, H.C. Tso, J. Dempsey, J. Hu, J. J. Palacios, J. Smoliner, J.J. Palacios, J.P. Eisenstein, J.P. Eisenstein, Jun Hu, K. Flensberg, K. Moon, K. Moon, L. Zheng, M. Buttiker, M. Wassermeier, M.A. Kastner, M.M. Fogler, O. Klein, P. Hawrylak, P.J. Price, P.L. McEuen, R.B. Laughlin, R.C. Ashoori, S.A. Govorkow, S.J. Allen, S.L. Sondhi, S.Q. Murphy, S.R. Eric Yang, S.S. Nazin, T.J. Gramila, T.J. Gramila, Tapash Chakraborty, U. Merkt, U. Sivan, V.A. Volkov, V.B. Shikin, X. G. Wen, X. G. Wen, X.G. Wen, X.G. Wen, X.G. Wen   +56 more
core   +3 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Double Reinforcement of Al–Fe Intermetallic Composites Fabricated by Friction Stir Processing

Metals, 2019
A double reinforced layer on an aluminum alloy surface was produced using friction stir processing (FSP) by adding 34CrNiMo6 powder into Al (AA2024) substrate for better wear resistance and gradient transitions. The microstructures of the composites were
Chunping Huang, Yang Xia, Chun Xia, Fencheng Liu   +3 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source