Results 71 to 80 of about 481,593 (267)

Effects of different types of fruit bags on the quality of Xiahui 10 peaches [PDF]

Guoshu xuebao
[Objective] Fruit bagging is a common practice used by growers to improve fruit quality, prevent pest and disease infestations, avoid direct pesticide contamination, enhance edibility, improve appearance, increase commercial value and raise market ...
ZHANG Binbin, DU Jinhua, CHEN Hong, WANG Xiaojun, ZHANG Yuanyuan, CHEN Zheng, DING Hui, YAN Zhimei, YU Mingliang, MA Ruijuan   +9 more
doaj   +1 more source

double-layer

, 2010
Citation: 'double-layer' in the IUPAC Compendium of Chemical Terminology, 3rd ed.; International Union of Pure and Applied Chemistry; 2006. Online version 3.0.1, 2019. 10.1351/goldbook.D01846 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for individual terms.
openaire   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

On the diffuse double layer [PDF]

Proceedings of the Royal Society of London. Series A, Containing Papers of a Mathematical and Physical Character, 1933
Abstract 1. The formulæ derived by Gouy for the diffuse double layer hold only in the case of a single surface in an infinite amount of medium. In practice they are not very suitable, for very frequently we have to deal either with capillaries, as in streaming potential measurements, or with colloidal particles which may be near ...
openaire   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Dynamic analysis on a buried horizontal double-layer SF-type liquidstorage tank by finite element method

Electronic Journal of Structural Engineering, 2018
To study the dynamic responses of a buried horizontal double-layer SF-type liquid-storage tank considering liquid-solid interaction, a three-dimensional finite element model of the double-layer buried horizontal liquid-storage tank is established, and ...
X. Cheng, L. Hu, W. Zhang, D. Yu
doaj   +1 more source

Mesoscopic Rough Electrical Double Layers

Physical Review Letters
Fundamental understanding of electrical double layers (EDL) has been gleaned mostly on ideally planar electrodes, while realistic electrodes usually exhibit surface roughness on multiple scales. The influence of mesoscopic roughness (1–10 nm) is elusive, representing a cutting-edge challenge to theoretical modeling as both quantum- and classical ...
Tang, Weiqiang, Liu, Jinwen, Doblhoff-Dier, Katharina, Huang, Jun   +3 more
openaire   +5 more sources

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source