Results 31 to 40 of about 52,924 (286)

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

open access: yesFEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil   +4 more
wiley   +1 more source

Sistemas de adubação de girassol e milho em consórcio de substituição Fertilization systems of sunflower and corn in relay intercropping

open access: yesCiência Rural, 1995
O consórcio,de substituição, em que a segunda cultura é implantada após a primeira ter atingido a antese, é um sistema que apresenta grande potencial de utilização na pequena propriedade.
Andréa Brondani da Rocha   +2 more
doaj   +1 more source

Toward in vitro fertilization in Brachiaria spp.

open access: yes, 2010
Brachiaria are forage grasses widely cultivated in tropical areas. In vitro pollination was applied to accessions of Brachiaria spp. by placing pollen of non-dehiscent anthers on a solid medium near isolated ovaries.
Willemse, M.T.M.   +6 more
core   +1 more source

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

Irrigation has more influence than fertilization on leaching water quality and the potential environmental risk in excessively fertilized vegetable soils. [PDF]

open access: yesPLoS ONE, 2018
Excessive fertilization is a common agricultural practice that often negatively influence soil and environmental quality in intensive vegetable production systems in China.
Yang Li   +3 more
doaj   +1 more source

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

open access: yesMolecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain   +10 more
wiley   +1 more source

Anatomical Study on the Multi-Ovule Development and Abortion of Hanfu Apple

open access: yesJournal of Integrative Agriculture, 2014
The terminal flower buds of 6-yr-old Hanfu apple were used to study the ovule development, ovular characteristics, cell death of abortive ovules, and dynamic change of starch grain quantity in the embryo sac with paraffin slices and terminal ...
Xu-yuan YANG   +5 more
doaj   +1 more source

A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose‐driven glioblastoma

open access: yesMolecular Oncology, EarlyView.
The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka   +9 more
wiley   +1 more source

Optimization Design and Experiment for Precise Control Double Arc Groove Screw Fertilizer Discharger

open access: yesAgriculture, 2023
In order to solve the problem of uniform and precise fertilizer discharge, based on experimental analysis of the uneven nature of single-screw fertilizer discharge, a double arc groove screw fertilizer discharger was designed based on the principle of ...
Guoqiang Dun   +4 more
doaj   +1 more source

Microarray analysis of single isolated cells of the female gametophyte reveals potential regulators of female germline development in Arabidopsis thaliana

open access: yes, 2013
This work has used single-cell isolation and transcriptome profiling to further our knowledge about the cells comprising the female gametophyte of Arabidopsis thaliana which have thus far been highly inaccessible.
Soljic, Lucija
core   +1 more source

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