Results 81 to 90 of about 3,153 (290)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

Toward a uniform account of scrambling and clitic doubling

open access: yes, 2008
A commonly held view in the literature on Scrambling and Clitic Doubling is that both constructions are sensitive to Specificity. For this reason Sportiche (1992) proposes to unify the two, an approach which has become quite standard in the relevant ...
Alexiadou, Artemis   +1 more
core  

Double Object Construction [PDF]

open access: yes, 2019
Tato bakalářská práce se zabývá ditranzitivními konstrukcemi, konkrétně vztahem mezi konstrukcí s dvojitým objektem a konstrukcí s předložkovým dativem.
Fridrich, Michal
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

LIGHT VERB CONSTRUCTIONS WHICH APPEAR IN THE DOUBLE OBJECT CONSTRUCTIONS AND IN THE PREPOSITIONAL DATIVE CONSTRUCTIONS IN ENGLISH. FOCUSING ON THE LIGHT VERB GIVE [PDF]

open access: yes, 2016
The aim of this paper is to analyse the behaviour of the light verb give, in English, that appears in the double object constructions and the prepositional dative constructions.
Carmen-Elena Stănculescu
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

open access: yesFEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer   +7 more
wiley   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

open access: yesFEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner   +7 more
wiley   +1 more source

Selective transfer in the acquisition of english double object constrctions by brazilian learners

open access: yesAlfa: Revista de Lingüística, 2013
The present study investigates the acquisition of the English double object constructions (GOLDBERG, 1995) by Brazilian learners. We hypothesize that, due to first language (L1) influences, the prepositional ditransitive construction (John gave a book to
Júlia Vidigal Zara   +2 more
doaj  

Replication Data for: A Probabilistic and Syntactic Account of Variable Clitic Agreement in Spanish Double Object Constructions

open access: yes
This is the data and R code to accompany the paper “A Probabilistic and Grammatical Account of Variable Clitic Agreement in Spanish Double Object Constructions”.
Guajardo, Gustavo
core   +1 more source

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