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Doubled Haploid Technology: Generation of Doubled Haploid Maize Lines Using Haploid Inducers
Cold Spring Harbor ProtocolsDoubled haploid (DH) technology allows for the development of completely homozygous lines from heterozygous plants in only two generations. This approach has been widely adopted in maize breeding programs, as it expedites the generation of inbred lines compared to traditional methods. The DH approach is based on the use of maize genotypes that have the
Vencke K. Grüning +2 more
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2022
Doubled haploid (DH) technology reduces the time required to obtain homozygous genotypes and accelerates plant breeding among other advantages. It is established in major crop species such as wheat, barley, maize, and canola. DH lines can be produced by both in vitro and in vivo methods and the latter is focused here.
Siddique I. Aboobucker +6 more
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Doubled haploid (DH) technology reduces the time required to obtain homozygous genotypes and accelerates plant breeding among other advantages. It is established in major crop species such as wheat, barley, maize, and canola. DH lines can be produced by both in vitro and in vivo methods and the latter is focused here.
Siddique I. Aboobucker +6 more
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Colchicine Alternatives for Chromosome Doubling in Maize Haploids for Doubled‐Haploid Production
Crop Science, 2016In vivo production of doubled‐haploid (DH) lines in maize (Zea mays L.) routinely involves artificial chromosome doubling by colchicine treatment of seedlings. Because colchicine is a hazardous chemical, replacing it by less‐toxic alternatives would be highly desirable.
Albrecht E. Melchinger +3 more
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2015
Climate changes, a rapid pathogen evolution, and market change, put breeders under constant pressure to deliver new and better varieties in a shorter time while simultaneously reducing the costs of variety development. Substantial progress has been generated in triticale doubled haploid (DH) technologies during the last decade that widens their ...
Maria Wędzony +5 more
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Climate changes, a rapid pathogen evolution, and market change, put breeders under constant pressure to deliver new and better varieties in a shorter time while simultaneously reducing the costs of variety development. Substantial progress has been generated in triticale doubled haploid (DH) technologies during the last decade that widens their ...
Maria Wędzony +5 more
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HAPLOID PRODUCTION AND CHROMOSOME DOUBLING
Acta Horticulturae, 2006In the applications of haploid plants, chromosome doubling has a major role and has been widely researched. Chromosome doubling restores fertility to plants and often results in genetically homozygous doubled haploids. In diploid and allopolyploid species these homozygous plants have the potential to become pure breeding new cultivars or the parents ...
K.J. Kasha +3 more
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2003
There are many factors influencing the deployment of doubled haploidy in breeding. Strategies for exploiting doubled haploids (DHs) vary within and among species and are dependent upon available technologies. Theoretical benefits and working protocols are not however sufficient to justify DH deployment in breeding.
W. T. B. Thomas +2 more
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There are many factors influencing the deployment of doubled haploidy in breeding. Strategies for exploiting doubled haploids (DHs) vary within and among species and are dependent upon available technologies. Theoretical benefits and working protocols are not however sufficient to justify DH deployment in breeding.
W. T. B. Thomas +2 more
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Haploids and doubled haploids in Citrus ssp.
2003Citrus breeding is based either on conventional (hybridization, selection, mutation) or biotechnological methods, the latter employing in vitro tissue culture, regeneration from protoplasts, somatic hybridization, in vitro mutant selection, genetic transformation and haploid/doubled haploid production. All cultivated forms of Citrus spp.
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Oat Doubled Haploids Following Maize Pollination
2017Doubled haploids (DHs) are an important tool for the accelerated production of new crop varieties. In oat, DHs were first produced by pollinating oat florets with maize pollen. The resultant embryos spontaneously eliminate the maize chromosomes leaving a haploid complement of oat chromosomes.
Philip A, Davies, Parminder K, Sidhu
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Theoretical and Applied Genetics, 1988
Different methods of line development using doubled haploids in recurrent selection are presented. They are divided into two types: recurrent selection with progeny testing and recurrent selection on the phenotype of lines. It is shown that one of the best methods of line development is based on “single doubled haploid descent recurrent selection ...
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Different methods of line development using doubled haploids in recurrent selection are presented. They are divided into two types: recurrent selection with progeny testing and recurrent selection on the phenotype of lines. It is shown that one of the best methods of line development is based on “single doubled haploid descent recurrent selection ...
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Rice Doubled Haploids and Breeding
2008Cell- and tissue culture methods in combination with conventional breeding process were suitable way to produce new varieties. These applications gave new breeding alternatives to release competitive genotypes in comparison with traditional ones. To the breeding of 'Risabell' (1997) DH lines were produced via anther culture from F 2 population of a ...
J. Pauk, M. Jancsó, I. Simon-Kiss
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